三唑锡亚致死浓度对土耳其斯坦叶螨运动速率的影响
Effects of sub-lethal concentrations of Azocyclotin on the movement rates of Tetranychus turkestani (Ugarov et Nikolskii)
刘 敏** 李永涛 李 婷 苏 杰 段祥坤 王振辉 张建萍***
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DOI：10.7679/j.issn.2095-1353.2015.069
作者单位：新疆石河子大学农学院，石河子 832000
中文关键词：三唑锡，亚致死浓度，土耳其斯坦叶螨，昆虫行为记录仪，运动速率
英文关键词：Azocyclotin, sublethal concentrations, Tetranychus turkestani, Track Sphere LC-100, motion rate
中文摘要：【目的】 通过研究三唑锡亚致死浓度LC10、LC20及致死中浓度LC50对土耳其斯坦叶螨Tetranychus turkestani运动速率的影响，为杀螨剂对土耳其斯坦叶螨在行为学方面影响提供科学依据，并将此作为评价药剂的作用机理的理论依据。【方法】 采用叶片浸渍法，明确致死中浓度和亚致死浓度，使用LC-100昆虫行为记录仪对土耳其斯坦叶螨在受到三唑锡亚致死浓度LC10、LC20和LC50刺激下其运动速率进行记录，观察其运动速率在三唑锡不同浓度、不同天数、不同时间段的变化规律。【结果】 在三唑锡不同亚致死浓度作用下，第1天到第3天的10:00―12:00，其运动速率比对照低，从第3天10:00―12:00期间到第5天20:00―22:00期间，其运动速率与对照没有显著性差异。在相同亚致死浓度作用下，第1天10:00―12:00期间，土耳其斯坦叶螨的运动速率显著高于15:00―17:00期间和20:00―22:00期间，而在第3天和第5天的3个时间段，对土耳其斯坦叶螨的运动速率没有显著影响。当作用的时段不同时，LC10第1天10:00―12:00显著高于第3天和第5天，而LC20、LC50在10:00―12:00没有显著性差异。三种浓度的第1天15:00―17:00显著低于第3天和第5天。第3及第5天20:00―22:00没有显著性差异。【结论】 总体来看，3唑锡亚不同致死浓度对土耳其斯坦叶螨的运动速率影响受浓度、时间影响，浓度越高土耳其斯坦叶螨运动速率越慢；随着时间延长，农药亚致死浓度对土耳其斯坦叶螨的运动速率基本没有影响。
英文摘要： [Objectives] To provide a scientific basis for the influence of Acraicides on the behavior of Tetranychus turkestani, and a theoretical basis for investigating the effect of sub-lethal (LC10, LC20), and lethal, concentrations (LC50) of Azocyclotin on the movement rates of T. turkestani. [Methods] The leaf immersion method was used to ascertain the median lethal concentration and sub-lethal concentrations of Azocyclotin. A tracksphere locomotion compensator with behavior recorder（Track Sphere LC-100）was used to record the movement rates of T. turkestani following exposure to the different sub-lethal concentrations of Azocyclotin (LC10, LC20 and LC50). Differences in movement rates following exposure to different concentrations of Azocyclotin, on different days and during different time periods, were analyzed. [Results] The results show that exposure to sub-lethal concentrations of Azocyclotin reduced movement rates relative to the control between 10:00 and 12:00 on the first and third day post-exposure. However, rates of movement were not significantly different from the control between 10:00 and 12:00 and between 20:00 and 22:00 on the third and fifth day. Exposure to sub-lethal concentrations increased the movement rate of T. turkestani on the first day between 10:00 and 12:00 compared to that between 15:00 and 17:00 and between 20:00 and 22:00. However, there was no significant difference in rate of movement during these three time periods on the third and fifth day. Exposure to the LC10 dosage significantly increased the movement rate of T. turkestani between 10:00 and 12:00 on the first day compared to that during the same period on the third and fifth days. Nevertheless, no significant difference was found between animals treated with LC20 and LC50. Exposure to all three sub-lethal concentrations significantly reduced movement rates between 15:00 and 17:00 on the third and fifth day. There was, however, no significant difference in movement rates between 20:00 and 22:00 on the third and fifth day. [Conclusion] The effects of sub-lethal concentrations of Azocyclotin on the movement rates of T. turkestani depended on pesticide concentration and time elapsed since exposure to the pesticide. The higher the sub-lethal concentration of Azocyclotin, the slower the movement rate of T. turkestani. However, this effect dissipated, and eventually became insignificant, over time.