不同温度效应杀虫剂诱导对绿盲蝽三种解毒酶活力的影响
The effect of temperature on the activity of three detoxification enzymes in Apolygus lucorum
刘 佳1, 2** 高占林1 党志红1 潘文亮1 袁文龙3 朱利红4 李耀发1***
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DOI：10.7679/j.issn.2095-1353.2015.071
作者单位：1. 河北省农林科学院植物保护研究所，河北省农业有害生物综合防治工程技术研究中心，农业部华北北部作物有害生物综合治理重点实验室，保定 071000；2. 河北农业大学植物保护学院，保定 071000；3. 河北省植保植检站，石家庄 050011； 4.高碑店园林局，高碑店 074000
中文关键词：解毒酶，绿盲蝽，温度效应，杀虫剂诱导，“钟形”曲线
英文关键词：detoxification enzymes, Apolygus lucorum, temperature effects, pesticide induction, "bell shaped" curves
中文摘要：
【目的】 为了明确解毒酶对绿盲蝽Apolygus lucorum防治药剂温度效应的影响机制。【方法】 本文测定了绿盲蝽3龄若虫体内3种主要解毒酶活力随温度变化的“钟形”曲线，以及在不同温度效应杀虫剂诱导后对绿盲蝽体内解毒酶“钟形”曲线的影响。【结果】 在供试温度10~40℃范围内，绿盲蝽体内3种主要解毒酶，在25℃时活力均最高，且显著高于其他温度处理。在不同温度下，4种不同温度效应杀虫剂诱导后对绿盲蝽体内解毒酶活力影响测定结果来看，谷胱甘肽-S-转移酶受杀虫剂诱导影响，可能参与了负温度效应杀虫剂溴虫腈的代谢，其在20~30℃的温度范围内，可能参与了吡虫啉和辛硫磷的部分代谢，而正温度效应杀虫剂氟铃脲在低温时毒力较低，可能与其诱导该酶在低温时活力升高相关。在15℃左右的低温时，绿盲蝽体内羧酸酯酶和多功能氧化酶易受氟铃脲和溴虫腈诱导而活力增加，而在20~35℃，这两种酶活力不易受杀虫剂诱导，且辛硫磷、溴虫腈和吡虫啉可对绿盲蝽体内两种酶活力产生一定的抑制作用。【结论】 绿盲蝽体内解毒酶活力可明显受到温度影响，而在杀虫剂诱导条件下，其可对绿盲蝽防治药剂的温度效应产生显著影响。
英文摘要：
[Objectives] To clarify the role of temperature on detoxification enzymes and pesticide resistance in Apolygus lucorum. [Methods] Temperature-dependent "bell shaped" curves of the three main detoxification enzymes of A. lucorum were measured, and the effects on these curves on different insecticides were determined. [Results] The results show that the activity of the three main detoxification enzymes are highest at 25℃, and significantly higher within the range of 10-40℃ than at other temperatures. The impact of detoxification enzymes of A. lucorum on insecticides that have temperature-dependent effects, indicate that the GST may be involved in the chlorfenapyr detoxification mechanism, the effectiveness of which is reduced by temperature in this insect. Between 20-30℃ the GST may be involved in the partial detoxification of imidacloprid and phoxim, and the low toxicity of hexaflumuron at low temperatures may also be correlated with this enzyme. CarE and MFO activity increased at about 15℃, and could be induced by the insecticides chlorfenapyr and hexaflumuron. Between 20-35℃, the activities of these two enzymes were not easily induced by the tested insecticides, but they could be inhibited by phoxim, chlorfenapyr and imidacloprid to some degree. [Conclusion] The results show that, after induction by pesticides, the activities of certain detoxification enzymes of A. lucorum are clearly affected by temperature, and that can have a significant influence on pesticide performance.