【目的】 昆虫血清素（5-羟色胺）受体已知有5个亚型。本文旨在系统分析昆虫5-羟色胺受体亚型蛋白的结构和进化关系。【方法】 首先对文献报道已明确亚型7种昆虫的5-羟色胺受体（23个亚型序列）进行生物信息学分析，然后采用多序列比对和进化树构建的方法对NCBI数据库中推测可能为昆虫5-羟色胺受体蛋白序列进行分析。【结果】 发现47个推测是昆虫5-羟色胺受体的蛋白序列中，有40个蛋白序列属于昆虫5-羟色胺受体，其余7个未能确认的昆虫5-羟色胺受体的蛋白序列都具有7个跨膜区域，属于G蛋白偶联受体家族，但不一定为5-羟色胺受体。【结论】本文对昆虫5-羟色胺受体蛋白的系统进化树分析，间接地证明了本文确认的昆虫5-羟色胺受体亚型注释信息的准确性，发现分类上同属一个目的昆虫5-HT受体序列的亲缘性较近。本研究为昆虫5-羟色胺受体的结构和功能分析提供基础。

  [Objectives]  There are five subtypes of serotonin receptor proteins of insects. The relationship between protein structure and the evolution of serotonin receptors in insects was comprehensively studied. [Methods]  First, the bioinformatics of 23 protein sequences of serotonin receptors in seven insects reported in the literature were analyzed; Second, the protein sequences of serotonin receptors in the NCBI database were verified by multiple sequence alignment and constructing a phylogenetic tree. [Results]  The results show that 40 of 47 putative protein sequences were those of insect serotonin receptors, whereas the other 7 protein sequences, which had 7 transmembrane regions, belong to the G protein-coupled receptor superfamily. It is uncertain if these 7 proteins are insect serotonin receptors. [Conclusion]  This study provides a basis for analyzing the function of insect serotonin receptors.