瓜实蝇耐低温相关生理指标的测定
Measurement of physiological indices of cold tolerance inBactrocera cucurbitae (Coquillett)
黄娜娜1, 2** 代 鹏2 符悦冠2 金 涛2*** 黄求应1***
点击：1584次 下载：30次
DOI：10.7679/j.issn.2095-1353.2015.081
作者单位：1. 华中农业大学湖北省昆虫资源利用与害虫可持续治理重点实验室，武汉 430070；2. 中国热带农业科学院环境与植物保护研究所，农业部热带农林有害生物入侵监测与控制重点开放实验室，海南省热带农业有害生物监测与控制重点实验室，儋州 571737
中文关键词：瓜实蝇，耐寒性，过冷却点，生理指标
英文关键词：Bactrocera cucurbitae (Coquillett), cold tolerance, supercooling point, physiological index
中文摘要：
【目的】了解瓜实蝇Bactrocera cucurbitae (Coquillett) 对低温的耐受性，分析其不同发育阶段耐寒力差异。【方法】 以不同发育阶段瓜实蝇为实验材料，分别测定并比较了过冷却点、水分、脂肪、甘油和糖类含量的变化情况。【结果】 其不同龄期的过冷却点差异显著，5日龄蛹的过冷却点最低，为 ﹣17.04℃，1日龄幼虫的过冷却点最高，为﹣11.82℃，同一日龄的雌、雄成虫之间的过冷却点无显著差异；其幼虫期和蛹期虫体的含水量随着龄期的增长而下降，从1日龄幼虫79.64%下降到5日龄蛹65.31%，5日龄蛹的含水量显著低于其他龄期瓜实蝇，成虫期各龄期雌成虫含水量均显著高于雄成虫；5日龄幼虫脂肪含量最高，为32.90%，1日龄雄成虫和14日龄雄成虫脂肪含量最低，均为15%；幼虫期甘油含量随着龄期的增长而下降，从3.32 μg/mg下降到1.12 μg/mg，而蛹期逐渐上升，14日龄雌成虫甘油含量最高，为5.90 μg/mg；1日龄幼虫总糖、海藻糖和糖原含量均最高，分别为7.51、0.94和1.93 μg/mg，显著高于其他龄期瓜实蝇，蛹期糖类含量逐渐下降，而成虫期糖类含量随龄期增长而逐渐升高。【结论】本研究结果可为正确评估瓜实蝇的地理适应性和低温检疫处理提供依据。
英文摘要：[Objectives] To investigate cold tolerance in different developmental stages of B. cucurbitae. [Methods] The supercooling point (SCP), and water, lipid, glycerin and carbohydrate content, of different developmental stages of B. cucurbitae, were measured and compared. [Results] Five day-old pupae had the lowest supercooling point of any developmental stage (﹣17.04℃) and 1 day-old larvae the highest (﹣11.82℃). However, there was no significant difference in the supercooling points of male and female adults of the same age. Water content reduced with age from 79.64% in 1 day-old larvae to 65.31% in 5 day-old pupae, which was the lowest water content recorded in all developmental stages. The water content of female adults were significantly higher than that of male adults in the same age. The fat content at 5 day-old larva was 32.90%, which was the highest among all the developmental stages. The lowest fat content were found in 1 day-old male adults (15%) and 14 day-old male adults (15%). The glycerol content in the larval stage decreased from 3.32 μg/mg to 1.12 μg/mg, and it increased gradually during the pupal stage. The highest point was detected in 14 day-old female adults (5.90 μg/mg). The total sugar content, trehalose content and glycogen content in 1 day-old larva all were the highest among all the developmental stages, which were 7.51, 0.94 and 1.93μg/mg respectively. The content of carbohydrates decreased gradually during the pupal stage, and they increased with the growth of age in the adult stage. [Conclusion] These results would provide the basis for exactly evaluating the geography adaptability and quarantine cold treatment on B. cucurbitae.