密粘褶菌松木粉培养物对黑翅土白蚁的生物活性
The biological activity of pine wood powder infected with Gloeophyllum trabeum on Odontotermes formosanus (Shiraki)
丁 芳1** 嵇保中1*** 刘曙雯2*** 张新慰1 杨锦锦1赵正萍1 王丽平1 王
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DOI：10.7679/j.issn.2095-1353.2015.086
作者单位：1. 南方现代林业协同创新中心，南京林业大学林学院，南京 210037；2. 南京中山陵园管理局，南京 210014
中文关键词：密粘褶菌，黑翅土白蚁，引诱活性物质，踪迹活性物质，提取液
英文关键词：Gloeophyllum trabeum (Pers.) Murrill, Odontotermes formosanus (Shiraki), attractive substance, trail-following substance, extracting solution
中文摘要：【目的】 研究密粘褶菌Gloeophyllum trabeum (Pers.:Fr.) Murr松木粉培养物对黑翅土白蚁Odontotermes formosanus (Shiraki)的生物活性。【方法】 比较了密粘褶菌松木粉培养物不同溶剂提取液对采食工蚁的踪迹和引诱活性，研究不同浓度正己烷和乙酸乙酯提取液的引诱活性变化。【结果】 正己烷提取液表现出高引诱活性和踪迹活性，乙酸乙酯、三氯甲烷、丙酮提取液具有高引诱活性和较低踪迹活性，甲醇、乙醇提取液表现出较高踪迹活性和较低引诱活性。正己烷提取液浓度与趋性反应率的回归方程为y = 12.561ln(x) + 50.11；乙酸乙酯提取液浓度与趋性反应率的回归方程为y = 12.161ln(x) + 51.411。 【结论】 密粘褶菌松木粉培养物对黑翅土白蚁采食工蚁具有明显的踪迹和引诱活性。正己烷和甲醇提取液的踪迹活性最强，乙酸乙酯和正己烷提取液的引诱活性最强。正己烷和乙酸乙酯提取液对采食工蚁的引诱活性具有很好的浓度相关性。
英文摘要：[Objectives] To study the attractivness of pine wood powder infected with Gloeophyllum trabeum (Pers.:Fr.) Murr to Odontotermes formosanus (Shiraki). [Methods] The attractiveness of extracts of pine wood powder infected with G. trabeum, and extracted with different solvents, to O. formosanus, was compared. Both general attractiveness and the rate of scent-trail following were assessed for each solvent. The effect of different concentrations of n-hexane and ethyl acetate extracting solutions on the attractiveness of extracts was also studied. [Results] n-hexane ranked highly in both general attractiveness and scent-trail following, whereas ethyl acetate, trichloromethane and acetone extracting solutions ranked highly in attractiveness but low in scent-trail following. Conversely, methanol and ethanol extracting solutions ranked high in scent-trail following but low in attractiveness. The linear relationship between n-hexane extracting solution concentration and scent-trail following rate can be expressed by the regression equation y = 12.561ln(x) + 50.11, and that between ethyl acetate extracting solution concentration and scent-trail following rate by y = 12.161ln(x) + 51.411. [Conclusion] Pine wood powder infected by G. trabeum is attractive to O. formosanus. n-hexane and methanol extracting solutions ranked highly in terms of scent-trail following and general attractiveness. There was a good correlation between the concentration of n-hexane and ethyl acetate extracting solutions and their attractiveness to O. formosanus.