刺吸电位技术实验中固定昆虫的三种方法比较研究
A comparative study of three methods using to fix insects for electrical penetration graph experiments
江守林** 何 花 屈 勇 陈法军***
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DOI：10.7679/j.issn.2095-1353.2015.126
作者单位：南京农业大学植物保护学院昆虫学系，昆虫信息生态研究室，南京 210095
中文关键词：刺吸电位技术，灰飞虱，昆虫固定技术，冷冻法，麻醉法，负压法
英文关键词：electrical penetration graph, Laodelphax striatellus, insect fixation technique, freezing method, anesthetization method, negative pressure method
中文摘要：
刺吸电位技术（Electrical penetration graph，EPG）是用来记录刺吸式口器昆虫的口针在其寄主植物组织中刺探所引起的电信号变化特征的技术，其核心在于建立昆虫取食行为与EPG波形的对应关系。近年来，EPG技术在昆虫取食行为及其传毒行为，以及植物抗虫机制等研究中得到越来越广泛的应用。其中，昆虫固定是影响EPG电极连接成功与否的关键技术，进而影响EPG实验结果。本文以灰飞虱Laodelphax striatellus（Fallén）为例，就EPG实验中电极连接时常用的3种昆虫固定技术（麻醉法、冷冻法和负压法）进行了详细描述与比较。研究结果表明，固定灰飞虱最优的方法为负压法，其次为麻醉法和冷冻法。
英文摘要：
Electrical penetration graph (EPG) is used to record the characteristics of electrical signal variation caused by sucking insects when their stylet pierces a host plant’s tissues. This can establish the correlation between an insect’s feeding behavior and EPG waveform types. In recent years, EPG has been widely used to study insect feeding behavior and virus transmission, and the mechanisms of host plants’ resistance to sucking insects. During EPG experiments, the method using to fix insect subjects is a pivotal skill that can affect the connectivity between the insect and the EPG electrodes. The small brown planthopper (SBPH), Laodelphax striatellus (Fallén) was used as a test insect to study and compare three commonly used fixation techniques (i.e., the negative pressure, anesthetization, and freezing, methods). On the basis of the results, these fixation techniques were ranked, in descending order; negative pressure method, anesthetization, freezing methods.