云南切梢小蠹气味结合蛋白的同源模建
Homology modeling of the odorant binding protein of the pineshoot beetle, Tomicus yunnanensis
汪 宇1** 孙 浩2 朱家颖1 赵 宁1 杨 斌1***
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DOI：10.7679/j.issn.2095-1353.2015.146
作者单位：1. 西南林业大学云南省森林灾害预警与控制重点实验室， 昆明 650224； 2. 西南林业大学理学院，昆明 650224
中文关键词：气味结合蛋白，同源模建，三维结构，云南切梢小蠹
英文关键词：odorant binding protein, homology modeling, three-dimensional structure, Tomicus yunnanensis
中文摘要：
【目的】 为了研究云南切梢小蠹Tomicus yunnanensis气味结合蛋白（Odorant binding proteins，OBPs）参与其嗅觉识别过程的机理奠定基础，本文对云南切梢小蠹OBP进行了同源模建及评价分析。 【方法】 采用同源模建方法构建了云南切梢小蠹OBP的三维结构，并利用Procheck、Verify_3D和ERRAT等方法评估了构建模型的可靠性。【结果】 对优化后的模建蛋白的结果分析发现，序列中有6个保守的半胱氨酸位点，具有气味结合蛋白的典型特征，并且Procheck、Verify_3D和ERRAT对模型的评价分数较高，结构具有很高的可能性。【结论】 基于已知的同源蛋白结构信息和模建蛋白的一级序列信息，预测序列已知但结构未知的蛋白空间结构，为今后研究该云南切梢小蠹气味结合蛋白的功能结构域奠定基础。
英文摘要： [Objectives] This study aims to provide the basis for revealing the mechanism of the odorant binding protein of the pine shoot beetle, Tomicus yunnanensis involved in olfactory reorganization [Methods] The three dimensional structure of T. yunnanensis’s OBP was constructed using the homology modeling method and the reliability of the model was evaluated by Procheck, Verify_3D and ERRATERRAT. [Results] Analysis of the optimized structure found that the OBP of T. yunnanensis contains 6 conservative cysteine residues in its sequence. Evaluation of the model by Procheck, Verify_3D and ERRATERRAT indicated that it is reasonable and reliable. [Conclusion] The results showed that it is possible to predict the structure of a protein that has a known sequence with unknown spatial structure based on known structural information of its homologous proteins. The results provide a foundation for further research on the functional structure domain of T. yunnanensis OBP.