交配对棉铃虫雌成虫抗菌肽表达水平的影响
The effect of mating on the expression of antimicrobial peptides in female Helicoverpa armigera
范荫荫** 杜孟芳 王 琼 刘孝明 安世恒 李为争 郭线茹 原国辉***
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DOI：10.7679/j.issn.2095-1353.2015.150
作者单位：河南农业大学植物保护学院，郑州 45000
中文关键词：棉铃虫，交配，抗菌肽，交配后反应，实时荧光定量PCR
英文关键词：Helicoverpa armigera, mating, antimicrobial peptide, postmating response, real time quantitative PCR
中文摘要：【目的】 探明交配对棉铃虫Helicoverpa armigera（Hübner）雌成虫抗菌肽表达水平的影响，为深入研究交配与雌成虫免疫反应的关系提供依据。【方法】 利用实时荧光定量PCR技术，分析了天蚕素3、天蚕素D、家蚕抗菌肽、攻击素、葛佬素、防御素和蚕幼抗菌肽共7个抗菌肽基因在棉铃虫处女雌虫和已交配雌虫腹部的表达情况。【结果】 结果表明，棉铃虫雌成虫交配后，除了天蚕素3基因表达量下降外，其他6种抗菌肽基因的表达量均显著上调，但表达高峰时间有所不同。其中葛佬素和蚕幼抗菌肽基因表达水平变化趋势相似，均在交配后1 h迅速达到高峰；家蚕抗菌肽、攻击素和防御素基因表达水平变化趋势相似，交配后表达量逐渐增加，到交配后3 h时达到高峰，此后开始下降；天蚕素D基因则在交配后1 h表达量没有明显变化，在交配3 h时显著增加，此后又逐渐减少。【结论】 除了天蚕素3基因外，6种供试抗菌肽基因表达量显著上调的高峰期均发生在雌成虫交配后3 h以内。
英文摘要： [Objectives] To investigate the effect of mating on the expression of antimicrobial peptides (AMPs) in female Helicoverpa armigera, and provide a basis for further research on the relationship between mating and female immune responses. [Methods] The relative expression of seven AMP genes, Cecropin 3, Cecropin D, Moricin, Attacin, Gloverin, Defensin and Lebocin, in the abdomens of virgin and mated H. armigera females were measured using real time quantitative PCR. [Results] With the exception of Cecropin D, expression of the other 6 genes generally increased within three hours of mating, although the timing of peak expression differed. The temporal trend in the expression of Gloverin and Lebocin was similar, with expression of both genes peaking just 1 h after mating. Temporal expression of Moricin, Attacin and Defensin was also similar, with expression increasing gradually to peak 3 h after mating, then declining. The expression of Cecropin D differed from that of the other genes in that there was no apparent change in expression 1 h after mating. Expression of this gene had obviously increased 3 h after mating, then gradually declined. [Conclusion] With the exception of Cecropin 3, expression of six other AMP genes generally occurred within 3 h of mating.