拟青霉菌代谢产物对菜粉蝶幼虫的生物活性
Insecticidal activities of metabolites from two Paecilomyces spp. to the small cabbage white, Pieris rapae
徐伟松1,2；罗建军1；麦燕玲1,3；李畅方2；钟国华1**
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作者单位：1华南农业大学昆虫毒理研究室 生物防治教育部工程研究中心 广州 510642；2广东省植物保护总站 广州 510500；3广东省江门市农产品质量监督检验测试中心江门 529000
中文关键词：拟青霉, 代谢产物，杀虫活性，菜粉蝶
英文关键词：Paecilomyces spp., metabolites, insecticidal activity, Pieris rapae
中文摘要：
淡紫拟青霉(Paecilomyces lilacinus)甲醇萃取物柱层析活性馏分DM6和拟青霉(Paeecilomyces sp.)菌株X1甲醇萃取物柱层析活性馏分X1M5对菜粉蝶Pieris rapae(L.) 4龄幼虫具有较强的选择性和非选择性拒食作用，处理后24 h选择性AFC50分别为5.865 2 mg/mL和3.523 9 mg/mL，非选择性AFC50分别为9.009 5 mg/mL 和 14.023 4 mg/mL。饲喂法、注射法、点滴法测定结果表明，DM6和X1M5对菜粉蝶4龄幼虫具有较强的毒杀活性，其中注射法处理后72 h，DM6和X1M5对菜粉蝶4龄幼虫的LD50分别为0.184 6 μg/头和0.678 4 μg/头。DM6和X1M5以5.0 mg/mL饲喂法处理菜粉蝶3龄幼虫后，试虫生长发育受到强烈的抑制作用，发育抑制率分别为73.34%和63.30%。这些结果对于进一步研究拟青霉菌杀虫活性成分具有重要参考意义。
英文摘要：The insecticidal activities of metabolites produced by Paecilomyces lilacinus and Paeecilomyces sp.X1 strain isolated from the infected aphids, against the small cabbage white, Pieris rapae (L.) (Lepidoptera: Pieridae) were reported in this paper. The methanol fractions were separated in the extensive separation by silica gel column and the subsequent biological activity tests showed that the 6th fraction of P.lilacinus（DM6） and the 5th fraction of Pieris sp. X1（X1M5） were their active fractions. The biological activity for P. rapae 4th instar larvae was investigated and the results showed that the AFC50 of DM6 and X1M5
were 5.865 2 mg/mL and 3.523 9 mg/mL in choice test and 9.009 5 mg/mLand 14.023 4 mg/mL in nochoice test after treatment 24 h, respectively. The active fractions DM6 and X1M5 may possessed strong toxicity against P. rapae 4th instar larvae by feeding, injection and topical application method, for example the LD50 of DM6 and X1M5 were 0.184 6 μg/larva and 0.678 4 μg/larva after treatment 72 h by injection method. The results showed the development inhibited rate (DIR) for 3rd instar larvae was 73.34% and 63.30% for DM6 and X1M5 respectively, by feeding contaminated leaves on the concentration of 5.0 mg/mL.