栎黄枯叶蛾生长发育与几种寄主内含物的关系
The effect of host plant species on the development of Trabalavishnou gigantina Yang (Lepidoptera: Lasiocampidae)
温冬梅1** 刘永华2 任利利1 陆鹏飞1 骆有庆1***
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DOI:10.7679/j.issn.2095-1353.2016.020
作者单位:1. 北京林业大学林木有害生物防治北京市重点实验室,北京 100083;2. 榆林学院生命科学学院,榆林 719000
中文关键词:寄主植物,栎黄枯叶蛾,生长发育,营养物质,次生物质
英文关键词:host plant, Trabala vishnou gigantina Yang, development, nutrients, secondary compounds
中文摘要:
【目的】 探讨饲喂不同寄主植物对栎黄枯叶蛾Trabala vishnou gigantina Yang生长发育及繁殖的影响,分析寄主植物内含物与其生长发育及繁殖的关系,为研究不同寄主植物对栎黄枯叶蛾种群动态的影响提供理论依据。【方法】 在室内条件下,利用沙棘Hippophae rhamnoides、山杏Armeniaca sibirica、山杨Populus davidiana、旱柳Salix matsudana饲养该虫,观察幼虫发育历期、蛹历期、蛹重及产卵量等指标,同时测定寄主叶片中可溶性糖、可溶性蛋白质、水分、单宁及黄酮的含量,并进行线性回归分析。【结果】 不同寄主植物饲喂栎黄枯叶蛾幼虫的发育历期、蛹历期、蛹重及产卵量均存在显著差异。取食沙棘的幼虫历期最短,仅为(74.50±1.76)d,而取食旱柳历期最长,达(106.00±1.51)d。取食山杨的蛹历期最长,为(44.00±1.41)d,最短的是取食旱柳的,仅为(32.70±3.13)d。饲喂沙棘和山杏的雌、雄蛹重和成虫产卵量显著高于饲喂山杨和旱柳的。对不同寄主的营养物质、次生物质和水分含量测定结果表明:不同寄主间营养物质、次生物质与水分的含量差异显著。回归相关分析表明:幼虫发育历期与寄主植物可溶性蛋白质含量呈显著负相关;雌蛹重和产卵量与寄主植物可溶性糖含量呈显著负相关;雌、雄蛹重与寄主植物含水量呈显著正相关;蛹历期与单宁含量呈显著正相关,但黄酮含量的高低与栎黄枯叶蛾生长发育等指标无相关性。【结论】 沙棘是栎黄枯叶蛾生长发育及繁殖的最适寄主植物;并且寄主植物中可溶性糖、单宁的含量低、含水量和可溶性蛋白质含量高有利于栎黄枯叶蛾的生长发育和繁殖。
英文摘要:
[Objectives] To examine the effects of feeding on different host plant species on the development of Trabala vishnou gigantina Yang, and determine the relative impact of different hosts on the population dynamics of T. vishnou gigantina. [Methods] The pupae weight, fecundity, and the duration of larvae and pupal stages of T. vishnou gigantina, reared on four different host plants (Hippophae rhamnoices, Armeniaca sibirica, Populus davidiana, Salix matsudana) under laboratory condition, were compared, and secondary compounds, soluble sugar, soluble protein and water content of the four host plants, determined. The relationship between larval development, adult fecundity, and host plant inclusion contents was analyzed. [Results] Host plant species had a significant effect on the duration of larval and pupal stages, pupal weight, and reproduction. Larvae fed on seabuckthorn had the shortest larval stage (74.50+-1.76) d, and those fed on willow the longest (106.00±1.51) d. Larvae fed on poplar had the longest pupal stage (44.00±1.41) d and those fed on willow the shortest (32.70±3.13) d. Larvae reared on seabuckthorn, or apricot, had significantly higher pupal weight and adult fecundity than those reared on poplar or willow. Nutrients, water and secondary compounds differed significantly between the different host plants. Soluble sugar content was significantly, negatively correlated with female pupal weight and fecundity, and soluble protein content was significantly negatively correlated with duration of the larval stage. Female and male pupal weights were positively correlated with water content. There was no relationship between flavone content and the duration of the larval stage or adult fecundity, but tannin content was significantly, positively correlated with the duration of pupal stage. [Conclusion] The results indicate that seabuckthorn was the most suitable of the four host plants for T. vishnou gigantina. The development and adult fecundity of T. vishnou gigantina are better when larvae feed on host plants with lower soluble sugar and tannin content, and higher water and soluble protein content.