飞蝗解毒酶系活力测定方法
Techniques for assaying detoxification enzyme activity in Locusta migratoria
董 玮** 吴海花 张建珍 王泽世 马恩波***
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DOI：10.7679/j.issn.2095-1353.2016.029
作者单位：山西大学应用生物学研究所，太原 030006
中文关键词：飞蝗，解毒酶系，非专一性酯酶，谷胱甘肽S-转移酶，细胞色素P450单加氧酶
英文关键词：Locusta migratoria, detoxification enzymes, non-specificesterases, glutathione S-transferases, cytochrome P450 monooxygenases
中文摘要：
飞蝗Locusta migratoria是重要的农业害虫，代谢抗性是飞蝗主要的农药抗性机制之一。与代谢抗性相关的解毒酶系主要有：非专一性酯酶系（Non-specficesterases，ESTs）、谷胱甘肽S-转移酶系（Glutathione S-transferases，GSTs）和细胞色素P450单加氧酶系（Cytochrome P450 monooxygenases，P450s），解毒酶系活力的测定是研究飞蝗农药代谢机制的重要途径。本文详细介绍了飞蝗解毒酶系的测定方法，为蝗虫及其他昆虫解毒酶系的测定提供参考。
英文摘要：
Locusta migratoria (L.) is a major agricultural pest and metabolic resistance is one of the most important mechanisms by which this species develops resistance to insecticides. The detoxification enzymes, which are associated with metabolic resistance, include nonspecific esterase (ESTs), glutathione S-transferases (GSTs) and cytochrome P450 monooxygenases (P450s). Assaying detoxification enzyme activity is an important way to study pesticide metabolism in L. migratoria. This paper describes in detail how to analyze the detoxification enzymes activity in L. migratoria, and provides a reference for this in other insects.