内参基因在昆虫实时荧光定量PCR中的研究进展
Advances in reference gene for real-time quantitative reverse transcription PCR (qRT-PCR) of insects research
史彩华1, 2** 张友军2***
点击：2202次 下载：80次
DOI：10.7679/j.issn.2095-1353.2016.031
作者单位：1. 长江大学，荆州 434025；2. 中国农业科学院蔬菜花卉研究所 北京 100081
中文关键词：昆虫，内参基因，qRT-PCR
英文关键词： insects, reference gene, real-time quantitative reverse transcription PCR
中文摘要：
作为一种高效的定量PCR技术，实时荧光定量PCR（qRT-PCR）因其灵敏度高、特异性强、定量准确等优点，已被广泛运用于昆虫基因表达和转录分析。然而，为了控制样本RNA在质量和逆转录效率上存在差异，必须筛选表达稳定的“看家基因”作为内参基因，对目的基因表达量进行校正和标准化。许多学者研究表明，昆虫种类和实验条件的不同，导致选择的内参基因也不尽相同。因此，本文综述了前人有关昆虫内参基因的研究及其稳定性评价，为其它昆虫内参基因的研究提供理论参考依据。
英文摘要：
Real-time quantitative reverse transcription PCR (qRT-PCR) is one of the most commonly used technologies for genes expression analysis, with its high sensitivity, specificity, veracity, etc. In order to avoid differences of RNA quality and efficiency of reverse transcription, it is necessary to use housekeeping gene as the reference for data normalization. Some researches indicate that different reference genes should be selected regarding different experimental conditions and different insect species. Here, we summarized the advances in reference genes for qRT-PCR of insects research. This review provides a comprehensive understanding of selecting appropriate reference genes for qRT-PCR in insect molecular biological studies.