
假眼小绿叶蝉对茶梢挥发物的行为反应
Behavioral responses of Empoasca vitis G?the tovolatiles from tea shoots
王梦馨 李辉仙 武文竹 孙海潮 石松平
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DOI:10.7679/j.issn.2095-1353.2016.066
作者单位:中国计量大学生命科学学院,浙江省生物计量及检验检疫技术重点实验室,杭州 310018
中文关键词:假眼小绿叶蝉,茶梢挥发物,引诱剂,行为反应
英文关键词:tea green leafhopper, tea shoot volatiles, attractant, behavioral responses
中文摘要:
【目的】 研究假眼小绿叶蝉Empoasca vitis Göthe对健康茶梢和蝉害茶梢挥发物的行为反应,筛选出可引诱假眼小绿叶蝉的茶梢挥发物。【方法】 以动态吸附法收集健康茶梢和蝉害茶梢挥发物,用气相色谱–质谱联用仪(GC-MS)进行定性定量分析,借助于Y形嗅觉仪检测多种挥发物引诱假眼小绿叶蝉成虫的活性。【结果】 从健康茶梢和蝉害茶梢中共鉴定出30种挥发物组分,其中烯烃类含量较大。健康茶梢和蝉害茶梢挥发物中共有组分有13种,蝉害之后其含量皆上升,其中Z-b-罗勒烯和乙酸叶醇酯的含量分别是健康茶梢中的142.27倍、12.90倍。蝉害茶梢中新出现的组分有12种,其中紫苏烯含量较高。在10-2、10-4、10-6 g/mL浓度下,乙酸叶醇酯表现出极显著的引诱水平(P<0.01);紫苏烯在10-2和10-4 g/mL浓度下表现出极显著引诱水平(P<0.01);10-4 g/mL浓度下,Z-b-罗勒烯和D-柠檬烯极显著引诱假眼小绿叶蝉(P<0.01);10-4 g/mL芳樟醇、10-6 g/mL乙酸正丁酯和10-6 g/mL D-柠檬烯呈现出显著引诱水平(P<0.05);混合物组分Blend1和Blend2分别表现出极显著和显著引诱水平;而10-6 g/mL a-法尼烯显著排斥假眼小绿叶蝉(P<0.05)。【结论】 假眼小绿叶蝉成虫对健康茶梢和蝉害茶梢挥发物多种组分具有不同的行为反应,引诱效果较强的单组分或混合组分的选定可为田间引诱效果试验提供参考。
英文摘要:
[Objectives] To determine the behavioral responses of the tea green leafhopper (Empoasca vitis Göthe) to volatile compounds emitted by both intact, and leafhopper-damaged, tea shoots in order to identify volatiles that attract this pest. [Methods] Volatiles from intact and leafhopper-damaged tea shoots were adsorbed by the routine overhead entrained air method, eluted in organic solvent, concentrated under a small flow of pure nitrogen, and qualitatively and quantitatively analyzed. The responses of leafhoppers to different volatiles were assessed in a Y-tube olfactometer. [Results] Thirty components in the volatiles of both intact, and leafhopper-damaged, tea shoots were identified in which the alkene content was relatively high. A total of 13 compounds were identified in the volatiles of intact and leafhopper-damaged tea shoots. The amounts of these compounds were considerably greater in volatiles emitted by leafhopper-damaged tea shoots than those emitted by intact tea shoots, especially (Z)-b-ocimene and (Z)-3-hexenyl acetate, which were respectively 142.27 and 12.90 times higher in volatiles from leafhopper-damaged tea shoots than in those from intact tea shoots. Twelve different compounds were identified in the volatiles of leafhopper-damaged tea shoots, among which perillene was particularly abundant. At concentrations of 10-2, 10-4, and 10-6 g/mL, (Z)-3-hexenyl acetate was significantly attractive to leafhoppers. Perillene was also significantly attractive at concentrations of 10-2 g/mL and 10-4 g/mL, (Z)- b-ocimene, as was (D)-limonene at 10-4 g/mL and 10-6 g/mL, linalool at 10-4 g/mL and n-butyl acetate at 10-6 g/mL. Two mixtures of different compounds were also significantly attractive, however, 10-6 g/mL of α-farnesene was not attractive to leafhoppers (P<0.05). [Conclusion] Adult tea green leafhoppers display different behavioral responses to components of the volatiles of intact tea shoots compared to those from leafhopper-damaged tea shoots. Identifying single component, or mixture of components, of volatiles that attract leafhoppers can provide a basis for field experiments.