温度胁迫对茶淡黄刺蛾四种保护酶活力和总抗氧化力的影响
Effect of temperature stress on four protective enzymes and overallantioxidant capacity in Darna trima (Moore)
李品武1** 付雪莲1, 2 陈世春2 胡 翔2 王晓庆2*** 彭 萍2***
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DOI：10.7679/j.issn.2095-1353.2016.100
作者单位：1. 四川农业大学园艺学院，成都 611130；2. 重庆市农业科学院茶叶研究所，永川 402160
中文关键词：茶淡黄刺蛾，温度胁迫，保护酶，总抗氧化能力
英文关键词：Darna trima, temperature stress, protective enzyme, total antioxidant capacity
中文摘要：
【目的】 温度适应性是决定昆虫分布和扩散的重要因素。为探索茶淡黄刺蛾Darna trima （Moore）对温度胁迫的耐受性，本研究测定了温度胁迫对4种抗氧化酶的活力和总抗氧化能力的影响。【方法】 分别将茶淡黄刺蛾老熟幼虫置于﹣5、0、5、26（对照）、35、37.5、40℃下处理2、4、6 h，测定其体内超氧化物歧化酶（SOD）、过氧化物酶（POD）、过氧化氢酶（CAT）、谷胱甘肽巯基转移酶（GST）和总抗氧化能力（T-AOC）的活力变化。【结果】 茶淡黄刺蛾的4种抗氧化酶的活力和总抗氧化能力在不同温度和时间之间均存在显著变化，但不同指标对温度胁迫具有不同的响应模式。低温胁迫尤其是﹣5℃对各指标影响最为显著，而高温胁迫影响较小。【结论】 SOD、CAT、POD、GST和T-AOC在茶淡黄刺蛾应对温度胁迫中具有重要作用，本研究可为判断茶淡黄刺蛾在茶区的分布范围提供一定的理论依据。
英文摘要：
[Objectives] Temperature adaptability is an important factor affecting the distribution and geographic spread of insects. To explore resistance to temperature stress in Darna trima (Moore), we investigated the activity of four protective enzymes and overall antioxidant capacity in this insect. [Methods] Mature larvae of D. trima were exposed to ﹣5, 0, 5, 26 (control), 35, 37.5 and 40℃ for 2, 4 and 6 h, and the activities of superoxide dismutase (SOD), catalase (CAT), peroxidase (POD), glutathione S-transferase (GST), and total antioxidant capacity (T-AOC), were measured. [Results] Different temperatures and exposure durations had significant effects on the activities of the four antioxidant enzymes and overall antioxidant capacity, and produced different response patterns. Cold stress, especially ﹣5℃, had significant effects on all indices, however, heat stress had relatively small effects. [Conclusion] SOD, CAT, POD, GST and T-AOC may play important roles in the response of D. trima to temperature stress. These results provide a theoretical basis for estimating the distribution of D. trima in tea plantations.