【目的】 克隆棉铃虫Helicoverpa armigera过氧化物酶基因（HaPOD）并进行序列分析，研究HaPOD基因的时空表达模式以及在极端温度、双氧水处理和HaNPV感染后的基因表达变化模式。【方法】 基于转录组测序获得HaPOD基因序列，通过几种生物信息学软件对该基因的核苷酸和氨基酸序列进行分析。采用实时荧光定量PCR技术检测HaPOD基因在棉铃虫体内的时空表达模式及4种逆境处理后的表达变化情况。【结果】 序列分析显示该基因开放阅读框长1 332 bp，编码443个氨基酸，含有一个细胞粘附蛋白序列，氨基酸序列与甜菜夜蛾Spodoptera exigua同源物序列一致性为85%，亲缘关系最近。HaPOD基因在5龄以前表达量相对较低，5龄后表达量开始升高，蛹第5天最高。在幼虫头和成虫翅中表达量相对较高。在高温、双氧水和HaNPV感染处理后，该基因表达量显著升高，而在低温处理后表达量显著下调。【结论】 本研究克隆得到了棉铃虫HaPOD基因序列并对其进行了分析，其表达量在高温、双氧水和HaNPV感染处理后上调而低温处理后下调，这些结果为进一步研究过氧化物酶在维持氧化还原平衡和抵抗氧化损伤方面的功能奠定基础。

[Objectives] To clone the HaPOD peroxidase gene in Helicoverpa armigera and analyze its function, including its temporal and spatial expression profiles, and determine its expression after injecting H₂O₂, exposure to extreme temperature and HaNPV infection. ［Methods］Full-length HaPOD cDNA was amplified using RT-PCR based on the transcriptome sequencing results for H. armigera. The gene and amino acid sequences of HaPOD were analyzed with several kinds of bioinformatics software. The spatio-temporal expression profiles of the HaPOD gene, and its expression patterns after 4 kinds of stress treatment (injection with H₂O₂, infection by HaNPV, and exposure to hot and cold temperatures) were determined by qRT-PCR. ［Results］Sequence analysis revealed that the ORF of the HaPOD gene was 1 332 bp and encoded 443 amino acids containing a peroxinectin sequence. The qRT-PCR results showed that the transcription level of HaPOD genes was low in larvae < 5 days of age, but increased to a maximum on the fifth day of the pupal stage. Spatial expression profiling indicates that the HaPOD gene was mainly expressed in the heads of larvae and in the wings of adults. Transcription of HaPOD markedly increased following injection with H₂O₂, HaNPV, or exposure to high temperature (35℃), but decreased following exposure to cold (4℃).［Conclusion］ The ORF of the HaPOD gene was successfully cloned and analyzed. Its expression was significantly upregulated by injection with H₂O₂, infection by HaNPV and exposure to high temperature, but was downregulated by cold temperature. These results provide a foundation for further research on the mechanisms involved in maintaining the redox balance and protection against oxidative damage.