取食行为对丽蝇蛹集金小蜂雌雄成虫体内 可培养真菌的影响
Effect of different foods on the fungal community cultured from adult female and male Nasonia vitripennis (Walker)
宋秀锋** 段入心** 王宁新***
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DOI：10.7679/j.issn.2095-1353.2017.055
作者单位：山东农业大学植物保护学院 山东省蔬菜病虫生物学重点实验室，泰安 271000
中文关键词：丽蝇蛹集金小蜂，可培养真菌，取食，性别，多样性
英文关键词： Nasonia vitripennis, culturable fungi, feeding, gender, diversity
中文摘要：
【目的】 本研究旨在阐明取食行为对丽蝇蛹集金小蜂Nasonia vitripennis（Walker）雌雄虫体内可培养真菌群落结构的影响，对其体内可培养真菌进行多样性研究。【方法】 采用不同浓度、不同培养基通过分离培养法研究丽蝇蛹集金小蜂体内真菌，将培养所得真菌采用形态和分子方法（ITS基因序列）进行鉴定。【结果】 取食前后丽蝇蛹集金小蜂雌、雄虫体内共分离得到49种真菌，隶属子囊菌门，盘菌亚门的座囊菌纲、散囊菌纲、锤舌菌纲、子囊菌纲，共10个属，其中以链格孢属（Alternaria）、枝孢属（Cladosporium）、青霉属（Penicillium）为优势属。【结论】 丽蝇蛹集金小蜂体内可培养真菌物种数目，雄虫多于雌虫，取食后多于取食前。本研究结果说明食物、性别是丽蝇蛹集金小蜂体内可培养真菌群落结构产生差异的重要因素。
英文摘要：
[Objectives] To clarify the effect of different foods on the fungal community of adult male and female Nasonia vitripennis (Walker). [Methods] We cultured fungi from N. vitripennis after feeding them with different concentration and dilution plate techniques, and different media, and quantified the results using both morphological and molecular (ITS gene) methods. [Results] Forty-nine different fungal species were isolated from male and female adults of N. vitripennis before and after feeding. These were classified into 4 classes (Dothideomycetes, Eurotiomycetes, Leotiomycetes, Ascomycetes) and 10 genera of Ascomycota. The predominant genera were Alternaria, Cladosporium and Penicillium. [Conclusion] Males had a greater number of fungal species than females, and more fungi were cultured after feeding than before. These results indicate that food and the gender are important factors affecting fungi that can be cultured from N. vitripennis.