谷蠹线粒体COⅠ基因序列及种群 遗传变异分析
Analysis of mitochondrial cytochrome oxidase Ⅰgene and genetic variation of Rhyzopertha dominica population
宋旭红1, 2** 王平坪1, 3 张宏宇1***
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DOI：10.7679/j.issn.2095-1353.2017.068
作者单位：1. 华中农业大学植物科学技术学院，武汉 430070； 2. 重庆市中药研究院，重庆 400065；3. 武汉工业学院，430023
中文关键词：谷蠹种群，线粒体DNA，细胞色素氧化酶Ⅰ基因，遗传分化
英文关键词：Rhyzopertha dominica population, mtDNA, cytochrome oxidase Ⅰ, genetic differentiation
中文摘要：
【目的】 对谷蠹的不同种群的线粒体COⅠ基因扩增片段进行比较，对谷蠹种群遗传结构进行深入分析。【方法】 对15个谷蠹种群的100个个体进行细胞色素氧化酶Ⅰ（COⅠ）基因部分序列进行分析。【结果】 得到691 bp长度的片段，3个位点的密码子中，A+T的含量平均值为63.2%，在3个密码子中的含量分别是85.2%，53.3%和57.0%。这种A+T含量的偏向在第1位点较第2和第3位点强。序列中A，T，C，G的含量分别为33.2%，32%，18.7%，16.2%。691 bp长度的碱基中，共发现26个多态位点，定义了25种单倍型，其中种群间共享单倍型有7个。【结论】 15个谷蠹种群之间存在一定的基因流（Nm = 1.35），使用AMOVA分析种群的遗传结构表明，谷蠹种群的变异主要发生在种群内部的个体间。
英文摘要：
[Objectives] To depth understanding the genetic structure by comparing the mtDNA-COⅠ gene fragment of different populations of Rhizopertha dominica (Fabricius). [Methods] The mtCOⅠ gene partial sequences (691 bp) of 100 individual from 15 R. dominica populations were amplified and sequenced. [Results] The molecular evolution characteristic of COⅠgene in different individuals was discussed. The results indicate that average A + T content of COⅠgene in R. dominica is 63.2%, which shows strong A + T bias. The A+ T content was 85.2%, 53.3% and 57.0% respectively in the three points. A + T content of this bias showed stronger in the first than that of the second and third points. A, T, C, G content of 33.2%, 32%, 18.7% and 16.2%, respectively. Twenty-six nucleotide sites were substituted and twenty-five heliotypes were identified in all sequences with seven shared. [Conclusion] The Nm value was 1.35 showed that there was certain gene flow among R. dominica population. AMOVA analysis of genetic structure indicated that the genetic variation of R. dominica mainly occurred among individuals.