基于线粒体DNA的宁夏、内蒙古及周边 地区棉铃虫种群遗传结构
Population genetic structure of Helicoverpa armigera in Ningxia, Inner Mongolia and neighboring areas
杨现明 陆宴辉**
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DOI：10.7679/j.issn.2095-1353.2018.004
作者单位：中国农业科学院植物保护研究所，植物病虫害生物学国家重点实验室，北京 100193
中文关键词：棉铃虫，线粒体基因，遗传结构，基因流，种群扩张
英文关键词：Helicoverpa armigera, mitochondrial gene, genetic structure, gene flow, population expansion
中文摘要：
【目的】 研究宁夏、内蒙古棉铃虫Helicoverpa armigera (Hübner)种群与华北、东北种群的遗传结构，以揭示宁夏、内蒙古两地虫源并为棉铃虫防治提供参考。【方法】 对采集得到宁夏银川、内蒙古巴彦淖尔等7个棉铃虫田间种群和1个银川室内种群共162个样本进行线粒体COⅠ基因片段（652 bp）测序并进行遗传分析。【结果】 分析显示共有23个COⅠ单倍型，其中H16和H23包含个体数量最多。宁夏、内蒙古田间种群与其他种群单倍型多样性（Hd）和核苷酸多态性（Pi）近似，与其他几个种群遗传分化（FST）较小，且不显著。种群间遗传距离和地理距离无显著相关性。中性测验分析显示混合和单个棉铃虫种群经历种群扩张。【结论】 宁夏、内蒙古种群与华北、东北种群存在着较强的基因流，可能主要由于棉铃虫兼性迁飞习性导致。
英文摘要：
[Objectives] The cotton bollworm, Helicoverpa armigera, caused significant damage to many crops in Ningxia and Inner Mongolia in 2017. To infer the origin of these pests in these two provinces, we studied the genetic structure of H. armigera in these provinces and in Northern and Northeastern China. [Methods] We sequenced and analyzed the mitochondrial DNA COⅠ gene fragments (652 bp) of 162 cotton bollworms from 7 populations in China. [Results] A total of 23 haplotypes were found, of which two source haplotypes (H16 and H23) were the most frequent. The haplotype diversity of all 7 populations was almost identical indicating that genetic differentiation between populations was low and non-significant. No significant correlation between genetic and geographic distances among populations was found. A neutrality test indicates that some populations may have experienced historical population expansion. [Conclusion] High gene flow, which may be due to migration, probably occurs between cotton bollworm populations in Ningxia, Inner Mongolia and Northern and Northeastern China.