玉米象Sitophilus zeamais DNA条形码研究
DNA barcoding of Sitophilus zeamais (Motschulsky)
陈光辉；李 焱；刘 东；李凤姐；阿依努尔·阿卜杜艾尼；胡红英
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DOI：10.7679/j.issn.2095-1353.2018.058
作者单位：新疆大学生命科学与技术学院，乌鲁木齐 830046；新疆维吾尔自治区喀什出入境检验 检疫局技术中心，喀什844000；3. 新疆石河子143团第一中学，石河子 832000
中文关键词：玉米象，DNA条形码，快速鉴定，ITS1，ITS2，Cytb，COⅠ，COⅡ基因
英文关键词：Sitophilus zeamais, DNA barcoding, rapid identification, ITS1，ITS2，Cytb，COI，COⅡ gene
中文摘要：
【目的】 玉米象Sitophilus zeamais（Motschulsky）为世界性分布仓储物害虫，食性极广，在我国多数省（区）均有分布。由于该虫与米象Sitophilus oryzae (Linnaeus)形态极似，很难区别；目前唯一可靠的鉴别特征是雌、雄外生殖器，且要实现准确鉴定必须要有成虫标本，还要经过一系列的繁琐操作，鉴定难度较大，因此需要探索更加便捷高效的鉴定技术。【方法】 利用分子生物学手段，通过提取玉米象的基因组DNA，使用通用引物PCR扩增ITS1、ITS2、Cytb、COⅠ、COⅡ基因并测序，通过基因序列相似性来实现快速鉴定，进而获得用于快速鉴定的DNA条形码。【结果】 通过形态特征，初步确定玉米象所属大致类群；通过分子生物学手段，获得了多个基因的碱基序列作为玉米象的DNA条形码。【结论】 获得多条序列用来鉴定玉米象的DNA条形码，实现玉米象快速准确鉴定，为玉米象的识别鉴定提供基础数据。
英文摘要：
[Objectives] Sitophilus zeamais (Motschulsky) is a global pest of a broad variety of stored foodstuffs that is found in most provinces (regions) of China. Many morphological features of S. zeamais are similar to those of S. oryzae, which makes these species difficult to distinguish reliably. Since the only diagnostic features are the male and female genitalia, it is only possible to reliably identify adults. It would be useful, therefore, to develop alternative methods that can efficiently and reliably identify other life-stages. [Methods] Genomic DNA of S. zeamais was extracted and the ITS1、ITS2、Cytb、COⅠ、COⅡ genes amplified and sequenced via PCR using universal primers. Rapid identification on the basis of gene sequence similarity was done and a DNA barcode for the rapid identification of S. zeamais obtained. [Results] S. zeamais specimens were identified based on morphological characteristics, and the base sequence of corresponding genes obtained and used as the DNA barcode for this species. [Conculsion] A number of gene sequences were obtained that can be used for DNA barcoding of S. zeamais, thereby allowing the rapid and accurate identification of this pest.