【目的】 研究替代寄主甜菜夜蛾Spodoptera exigua增殖的Se-DpCPV及其复合剂对马尾松毛虫Dendrolimus punctatus的防治效果，为进一步研究马尾松毛虫林间大规模防治提供依据。【方法】 在室内利用接种不同浓度病毒的松针饲喂马尾松毛虫幼虫，比较替代寄主增殖的Se-DpCPV和野生型DpCPV毒力能力以及Se-DpCPV对不同龄期马尾松毛虫幼虫毒力水平；同时，在林间利用固定翼植保无人机喷洒不同浓度DpCPV及其复合剂，测定各组对第1代马尾松毛虫幼虫的防治效果。【结果】 甜菜夜蛾增殖的Se-DpCPV和野生型DpCPV对马尾松毛虫幼虫具有同样的感染致病能力，对马尾松毛虫3龄幼虫半数致死浓度LC₅₀分别为4.73×10³ PIB/mL和3.36×10³ PIB/mL，二者毒力差异性不显著（P=0.107＞0.05）。Se-DpCPV对马尾松毛虫2-5龄各龄幼虫半数致死浓度LC₅₀分别为2.03×10³、4.73×10³、1.05×10⁴、3.85×10⁴ PIB/mL。当Se-DpCPV林间用量分别为375 亿、750 亿和1 500 亿PIB/hm²时，Se-DpCPV+3.2%阿维菌素复合剂防治组马尾松毛虫校正死亡率为86.05%、90.70%和94.19%，对马尾松毛虫的林间防治效果显著高于Se-DpCPV+25%灭幼脲复合剂（66.28%、72.09%和79.07%）和Se-DpCPV（59.30%、63.95%和70.93%）。活虫DpCPV感染率最低为80.00%，最高可达93.33%。Se-DpCPV与3.2%阿维菌素、25%灭幼脲复配，比单独使用两种药剂防治效果至少分别提高17.45%和33.72%。【结论】 在林间马尾松毛虫大暴发时，在马尾松毛虫3龄以下幼虫期，使用DpCPV+3.2%阿维菌素复合剂（750 亿PIB/hm²，7.5 mL/hm²）在短时间内迅速降低虫口基数，同时起到持续控制的效果。

 [Objectives]  To provide a foundation for further research on the bio-control of Dendrolimus punctatus in the field. The virulence of the Dendrolimus punctatus cytoplasmic polyhedrosis virus (DpCPV) propagated in the substitute host Spodoptera exigua, named Se-DpCPV, and its compound agent, to D. punctatus, were studied. [Methods]  The virulence of Se-DpCPV to D. punctatus larvae was compared to that of the original isolate DpCPV by feeding larvae pine needles infected with different concentrations of the virus, and the virulence of Se-DpCPV to D. punctatus larvae of different ages was determined. A field trial of the effectiveness of spraying different concentrations of Se-DpCPV and its compound agents from a fixed-wing unmanned aerial vehicle to control first generation of D. punctatus larvae was also conducted. [Results]  Se-DpCPV was as infectious as the original isolate to 3^rd instar larvae of D. punctatus and its median lethal concentration (LC₅₀) of 4.73×10³ PIB/mL was not significantly different from that of the original isolate (3.36×10³ PIB/mL). Median lethal concentrations (LC₅₀) of Se-DpCPV against 2^nd, 3^rd, 4^th and 5^th instar larvae of D. punctatus were 2.03×10³, 4.73×10³, 1.05×10⁴ and 3.85×10⁴ PIB/mL, respectively. Spraying 3.75×10¹⁰, 7.50×10¹⁰ and 1.50×10¹¹ PIB/hm² Se-DpCPV mixed with 3.2% avermectin (7.5 mL/hm²) achieved corrected mortality rates of D. punctatus of 86.05%, 90.70% and 94.19%, respectively. The control efficiency of this mixture was significantly higher than that of 25% chlorbenzuron (1 g/hm²), for which the corresponding corrected mortality rates were 66.28%, 72.09% and 79.07%. Corrected mortality rates for three different concentrations of Se-DpCPV applied without an insecticide were 59.30%, 63.95% and 70.93%. The infection rate of D. punctatus was 80.00% to 93.33%. Compared with 3.2% avermectin and 25% chlorbenzuron, the control efficiency of the Se-DpCPV mixture was at least 17.45% to 33.72% higher. [Conclusion]  A mixture of Se-DpCPV (7.50×10¹⁰ PIB/hm²) and 3.2% avermectin (7.5 mL/hm²) is recommended for the control of D. punctatus before the 3^rd instar. This mixture can reduce the population base of D. punctatus within a short time and has a long-lasting effect.