埃及伊蚊表皮结构蛋白AaCPR100A的抗原表位的生物信息学预测
Bioinformatic prediction of epitopes of the cuticular gene AACPR100A in Aedes agypti
陈 静** 卢浩然** 张 磊 廖承红*** 韩 谦***
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DOI：10.7679/j.issn.2095-1353.2019.035
作者单位：海南大学生命科学与药学院，海口 570228
中文关键词：埃及伊蚊；抗原表位；DNAStar 8；AaCPR100A
英文关键词：Aedes aegypti; epitope; DNAStar software; AaCPR100A
中文摘要：
【目的】 预测埃及伊蚊Aedes aegypti表皮结构蛋白AaCPR100A的抗原表位。【方法】 通过DNAStar 8的Protean软件对埃及伊蚊表皮结构蛋白AACPR100A的二级结构和亲疏水性、表面可及性、柔韧性等特性进行分析，预测其潜在的B细胞抗原表位和潜在的T细胞抗原表位。【结果】 AaCPR100A蛋白的二级结构以转角和不规则卷曲为主，表面可及性、柔韧性较强，存在7个潜在的B细胞抗原表位，位于18-29、32-42、47-82、87-98、111-178、194-248氨基酸残基或其附近，同时有11个潜在的T细胞抗原表位，位于20-24、35-39、44-48、51-53、79-82、103-106、109-112、146-148、150-152、185-203、224-235氨基酸残基或其附近。【结论】 AaCPR100A蛋白潜在的B细胞抗原表位有7个，潜在的T细胞抗原表位有11个，综合后有3个潜在抗原表位，预测结果将为进一步研究AaCPR100A蛋白的免疫学特性和功能奠定基础，并为蚊虫的控制提供潜在的靶标。
英文摘要：
[Objectives] To predict the epitopes of the Aedes aegypti epidermis protein AaCPR100A. [Methods] The secondary structure and hydrophobicity, surface probability and flexible regions of AaCPR100A were analyzed by DNAstar software to predict potential B cell antigen epitopes and potential T cell antigen epitopes. [Results] The secondary structure of the AaCPR100A protein was rich in β-turns and random coils, and had high surface probability and flexibility. There were 7 potential B cell epitopes located at amino acid residues 18-29, 32-42, 47-82, 87-98, 111-178 and 194-248, or nearby, and also 11 potential T cell epitopes located at amino acid residues 20-24, 35-39, 44-48, 51-53, 79-82, 103-106, 109-112, 146-148, 150-152, 185-203 and 224-235, or nearby. [Conclusion] The AaCPR100A protein contains both potential B cell antigen epitopes and potential T cell antigen epitopes. The predicted results provide a foundation for studies of the immunological properties and function of the AACPR100A protein, and suggest a potential target protein for mosquito control.