酪蝇雌虫卵巢提取物对其雄虫的引诱效果
The effectiveness of using ovarian extract from the female cheese skipper, Piophila casei, as a lure to trap males of this species
李 颖** 戚敬威 熊 焰 钱晓香 苏发武 肖 春 董文霞***
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DOI：10.7679/j.issn.2095-1353.2019.040
作者单位：云南农业大学植物保护学院，昆明 650201
中文关键词：酪蝇；卵巢提取；引诱；性信息素
英文关键词：College of Plant Protection, Yunnan Agricultural University, Kunming 650201, China
中文摘要：【目的】 本文研究目的是验证酪蝇Piophila casei雌虫卵巢是否存在性信息素，并为酪蝇性信息素研究提供科学依据。【方法】 测定了酪蝇雄虫对不同剂量的雌虫卵巢提取物的行为选择反应和触角电位反应，还利用模拟仓储实验测定了不同剂量的雌虫卵巢提取物对酪蝇雄虫的诱捕效果。【结果】 当酪蝇雌虫卵巢提取物的剂量分别为0.5、1、1.5、2、2.5头当量时，雄虫对0.5、1、1.5头当量的雌虫卵巢提取物有选择反应，雄虫对这5个剂量的雌虫卵巢提取物均具有触角电位反应。酪蝇雌虫卵巢提取物对雄虫诱捕效果的模拟仓储实验结果显示：当酪蝇卵巢提取物的剂量分别为2、4、6、8、10头当量时，在非选择性实验中各当量的雌虫卵巢提取物对雄虫均有引诱效果，在选择性实验中雌虫卵巢提取物的剂量为2头当量时，对雄虫的引诱效果最好。【结论】 酪蝇雌虫卵巢提取物对雄虫有显著的引诱效果，表明酪蝇雌虫卵巢中存在吸引雄虫的化学成分，即酪蝇的性信息素。
英文摘要： [Objectives] To investigate whether sex pheromones extracted from the ovary of the cheese skipper, Piophila casei, are effective lures for trapping males of this species. [Methods] The behavioral and electroantennogram (EAG) responses of male cheese skippers to ovarian extracts were measured, and an experiment comparing the effectiveness of different trapping lures was conducted in a simulated warehouse. [Results] Significantly more male cheese skippers choose the ovarian extract than the control (acetone). EAG results indicate that all tested doses of the extract elicited EAG responses from males, and all tested doses trapped more males than the control in a non-choice experiment. A choice experiment indicated that a dose equivalent to the amount of pheromone obtained from 2 females was the most attractive to males. [Conclusion] The ovarian extract of female cheese skippers was significantly more attractive to males than a control substance.