
温度胁迫对腐食酪螨抗氧化能力的影响
Effect of temperature stress on the antioxidant capacity of Tyrophagus putrescentiae (Schrank)
王 静; 阙生全;奚剑飞;邹志文;辛天蓉; 夏 斌
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DOI:10.7679/j.issn.2095-1353.2019.079
作者单位:南昌大学生命科学学院,南昌 330031;江西省林业科学院,南昌 330013
中文关键词:腐食酪螨;温度胁迫;抗氧化酶活力
英文关键词: Tyrophagus putrescentiae; temperature stress; antioxidant enzyme activity
中文摘要:【目的】 以储藏物主要害螨腐食酪螨Tyrophagus putrescentiae(Schrank)为研究对象,采用温度胁迫处理,探讨其体内抗氧化系统对不同温度胁迫的响应机制。【方法】 采用低温(0、5、10、15、20 ℃)、常温(25 ℃)、高温(30、33、36、39、42和45 ℃)胁迫处理1、2、3 h后,测定腐食酪螨超氧化物歧化酶(SOD)、过氧化物酶(POD)和谷胱甘肽-S-转移酶(GSTs)3种酶的活力以及总抗氧化(T-AOC)能力和丙二醛(MDA)含量的变化。【结果】 腐食酪螨3种抗氧化酶的活力、总抗氧化能力和MDA含量变化都与胁迫温度和时间密切相关;无论低温还是高温胁迫,SOD酶和GSTs酶活性较对照组均显著增加,说明它们在清除活性氧(ROS)过程中发挥着重要作用。【结论】 温度胁迫对腐食酪螨有重要作用,可提高其抗氧化酶活力。
英文摘要:[Objectives] To investigate the antioxidant system of the storage pest mite Tyrophagus putrescentiae under different temperature stress treatments. [Methods] The activity of superoxidedismutase (SOD), peroxidase (POD), glutathione-S-transferase (GSTs), total antioxidant (T-AOC) capacity and malondialdehyde (MDA), were measured after 1, 2 and 3 hours exposure to low (0, 5, 10, 15, 20 ℃), control (25 ℃), or high (30, 33, 36, 39, 42, 45 ℃), temperatures. [Results] The activities of all three antioxidant enzymes, total antioxidant capacity and MDA content were closely related to both temperature and the duration of exposure. SOD enzyme and GSTs increased significantly under the low and high temperature treatments, indicating that temperature plays an important role in eliminating ROS. [Conclusion] The activity of antioxidant enzymes in T. putrescentiae were affected by temperature stress.