基于代谢组和转录组的白蜡虫吊糖成分及其作用分析
The composition and function of the honeydew secreted by Ericerus pela as deduced from metabolome and transcriptome data
赵遵岭；于淑惠；周文武； 祝增荣；郭忠建； 杨 璞
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DOI：10.7679/j.issn.2095-1353.2019.111
作者单位：1. 中国林业科学研究院资源昆虫研究所，国家林业局资源昆虫培育与利用重点实验室，昆明 650224； 2. 昆明学院农学与生命科学学院，昆明 650224；3. 浙江大学昆虫科学研究所，水稻生物学国家重点实验室， 杭州 310058； 4. 江苏大学生命科学研究院，镇江 212000
中文关键词：白蜡虫；吊糖；转录组分析；代谢组学分析；基因家族
英文关键词：Ericerus pela; honeydew; transcriptome analysis; metabolomics analysis; gene family contraction
中文摘要：【目的】 白蜡虫Ericerus pela Chavannes在育卵期会大量分泌吊糖（蜜露），前期研究发现吊糖容易受到真菌的侵染，但是白蜡虫吊糖的生物学意义并不清楚。本研究旨在了解白蜡虫吊糖的成分以及该时期雌成虫基因表达特点，为更深入的了解白蜡虫分泌吊糖对于白蜡虫发育的意义奠定基础。【方法】 对吊糖分泌期的白蜡虫雌虫进行转录组测序分析，对吊糖组分进行代谢组学分析，并对转录组与代谢组进行联合分析。【结果】 在白蜡虫吊糖组分中，麦芽糖醇含量最高，其次为半乳糖醇，之后为海藻糖、水苏糖等其他种类糖物质，此外，还含有多种少量氨基酸、脂肪、维生素等代谢物质或次生代谢物质；关联分析表明，代谢途径（Metabolic pathways）、碳代谢（Carbon metabolism）和氨基酸合成（Biosynthesis of amino acids）占主要部分；与N-聚糖生物合成（Various types of N-glycan biosynthesis）相关通路的基因家族在白蜡虫发生了收缩；与代谢产物关联的基因Q96JB1家族也发生了收缩，该基因与吊糖中的L-谷氨酸、乳清酸盐以及柠檬酸盐相关。【结论】 白蜡虫吊糖主要成分不利于天敌寄生或者与其它昆虫形成互利共生，一些与吊糖成分相关的基因家族在白蜡虫中发生了收缩，说明白蜡虫吊糖可能是其分泌的不利的或多余的代谢物。
英文摘要：[Objectives] Female Ericerus pela can secrete a considerable amount of honeydew during the incubation period. We previously found that this honeydew was prone to contamination by certain fungi. However, the biological significance of the honeydew secreted by female E. pela is still largely unclear. This study aimed to understand the composition of the honeydew secreted by E. pela, and provide an overview of gene expression in female adults during this period. We hope the results will lead to a deeper understanding of the biological significance of honeydew secretion by E. pela. [Methods] The transcriptome of incubating female E. pela period was sequenced and the metabolome of the honeydew secreted by females was analyzed. [Results] The single greatest component of honeydew was maltitol, followed by galactositol, trehalose, triose and other sugars. Honeydew also contained a small amount of various amino acids, fats, vitamins, and other metabolites or secondary metabolites. Combining the transcriptome and metabolomic data revealed that the main pathways were “metabolic”, “carbon” and “biosynthesis of amino acids”. The gene family related to ‘N-glycan biosynthesis’, and another containing the gene Q96JB1 with the corresponding metabolites citrate, orotate and L-glutamate, were contracted. [Conclusion] The main components of the honeydew secreted by E. pela are not beneficial for parasitic biological control agents or for mutualism with other insects. Some gene families related to the components of honeydew have been contracted. These results show that the honeydew secreted by E. pela contains unnecessary metabolites, or may be harmful to female E. pela.