不同行为表现的蜜蜂脑部多巴胺 及其受体基因的检测分析
The amount of dopamine, and the expression of its receptor genes, in the brains of worker honey bees engaged in different activities
张曌楠；黄景南；封王江；余岢骏；李志国；聂红毅；苏松坤
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DOI：10.7679/j.issn.2095-1353.2019.140
作者单位：福建农林大学动物科学学院（蜂学学院），福州 350002；福建农林大学动物科学学院（蜂学学院），福州 350002；福建农林大学动物科学学院（蜂学学院），福州 350002；福建农林大学动物科学学院（蜂学学院），福州 350002；福建农林大学动物科学学院（蜂学学院），福州 350002；福建农林大学动物科学学院（蜂学学院），福州 350002；福建农林大学动物科学学院（蜂学学院），福州 350002
中文关键词：意大利蜜蜂；多巴胺；神经递质；高效液相色谱-电化学检测；基因；行为
英文关键词：Apis mellifera ligustica; dopamine; neurotransmitters; high performance liquid chromatograph-electrochemical detection system; gene; behaviors
中文摘要：
【目的】 对不同行为表现的意大利蜜蜂Apis mellifera ligustica 工蜂脑部多巴胺及其受体基因进行检测。【方法】 从蜜蜂观察箱中采集不同行为表现的工蜂，解剖其脑部，用高效液相色谱-电化学检测器和实时荧光PCR仪分别检测多巴胺含量和受体基因相对表达量，并进行计算分析研究。【结果】 建立了不同行为表现的蜜蜂脑部多巴胺及受体基因的研究方法。意大利蜜蜂哺育蜂脑部的多巴胺含量与新出房工蜂、采集蜂、休息状态下的工蜂存在显著差异。多巴胺受体1基因及多巴胺转运体基因在哺育蜂脑部处于高表达状态，4种不同分工的意大利蜜蜂脑部多巴胺受体2基因、受体3基因相对表达量差异不显著。【结论】 多巴胺与蜜蜂不同行为表现密切相关，多巴胺受体1基因与转运体基因可能参与蜜蜂哺育行为。
英文摘要：[Objectives] To quantify the
amount of dopamine, and the expression of its receptor genes, in the brains of
worker bees (Apis mellifera ligustica) engaged in different activities.
【Methods】 Worker
bees performing different activities were collected from a bee observation
hive, and their whole brain dissected and removed. The dopamine content was
measured with a high performance liquid chromatography-electrochemical detector
and the relative expression of its receptor genes with a Quantitative Real-time
PCR instrument.【Results】 A method for measuring dopamine
and the expression of its receptors gene in the brains of honeybees with
different roles was established. The dopamine content in the brain of nurse honeybees
was significantly different from that of newly emerged, foraging or resting,
workers . Furthermore, the dopamine receptor gene 1 and transporter genes were
more highly expressed in nurse bees. However, the relative expression level of
the dopamine receptor genes 2 and 3 in the brains of workers engaged in these
four different activities was not significantly different.【Conclusion】 Dopamine is closely related to the behavior
of bees; the dopamine receptor 1 gene and transporter genes may be involved in feeding
behavior.