【目的】 分析蜂王上颚腺信息素（Queen mandibular pheromone，QMP）对中华蜜蜂Apis cerana cerana（简称中蜂）雄蜂与意大利蜜蜂Apis mellifera ligustica（简称意蜂）雄蜂行为反应的变化，探索两蜂种雄蜂之间婚飞的干扰机理。【方法】 本试验通过室内Y型嗅觉仪检测QMP及其主要成分反式-9-氧代-2-癸烯酸[(E)-9-oxodec-2-enoic acid，9-ODA]对飞行、爬行状态下中蜂雄蜂与意蜂雄蜂选择行为的差异进行研究。【结果】 飞行或爬行状态下的中蜂雄蜂和意蜂雄蜂，均对3.5、7.0和14.0 μg/μL 的9-ODA没有显著趋向性反应（P > 0.05）；飞行中蜂雄蜂、飞行与爬行意蜂雄蜂均对0.04、0.2、1.0及7.0 μg/μL的QMP具有显著趋避反应（P < 0.05），而QMP对爬行中蜂雄蜂无显著影响（P > 0.05）；在中蜂雄蜂和意蜂雄蜂共存时的试验中发现：飞行、爬行意蜂雄蜂均对7.0 μg/μL QMP存在显著趋避反应（P < 0.05），而7.0 μg/μL QMP对飞行或爬行中蜂雄蜂均无显著影响（P > 0.05）。【结论】 在室内环境下，QMP对中蜂、意蜂雄蜂有驱避作用。

[Objectives]  To analyze the behavioral effect of queen mandibular pheromone (QMP) on Apis cerana cerana and Apis mellifera ligustica drones, and to investigate the drone mating interference mechanism of these two species. [Methods]  The behavioral responses of mature flying or crawling A. mellifera and A. cerana drones to QMP and (E)-9-oxodec-2-enoic acid (9-ODA, the predominant compound of QMP), were measured using an indoor olfactometer. [Results]  Exposure to three different doses of 9-ODA (3.5, 7.0 and 14.0 μg·μL^﹣1) had no significant effect on the behavior of either A. mellifera and A. cerana drones, but QMP at concentrations of 0.04, 0.2, 1.0 and 7.0 μg·μL^﹣1 had a significant effect on flying A. cerana drones, and on crawling and flying A. mellifera drones, but no significant effect on crawling A. cerana drones. When drones of both species were placed together in the indoor olfactometer 7.0 μg·μL^-1 QMP had a significant effect on both flying and crawling A. mellifera drones, but no significant effect on flying or crawling in A. cerana drones. [Conclusion]  This study suggests that QMP has a repellent effect on A. cerana and A. mellifera drones in the indoor environment.