
外源海藻糖对水稻生理生化及褐飞虱抗性的影响
Effects of trehalose on the physiological and biochemical characteristics of rice, including resistance to the brown planthopper (BPH), Nilaparvata lugens (St?l) (Hemiptera: Delphacidae)
孙李曈;冯 玲;刘子睿;徐小伟;刘景澜
点击:1594次 下载:62次
DOI:10.7679/j.issn.2095-1353.2020.083
作者单位:扬州大学园艺与植物保护学院;扬州大学园艺与植物保护学院;扬州大学园艺与植物保护学院;扬州大学园艺与植物保护学院;扬州大学园艺与植物保护学院
中文关键词:海藻糖;水稻;生理生化;褐飞虱;抗虫性
英文关键词:College of Horticulture and Plant Protection, Yangzhou University
中文摘要:
【目的】 海藻糖参与植物对逆境胁迫的响应与适应过程,本文旨在明确海藻糖对水稻生理生化特性及抗褐飞虱影响,将有助于全面探索海藻糖对水稻的潜在作用,为后续研究提供参考和依据。【方法】 本实验在外施10 mmol∙L-1和50 mmol∙L-1浓度的海藻糖后,测定水稻超氧化物歧化酶(SOD)、过氧化物酶(POD)活性,丙二醛(MDA)和可溶性糖含量,褐飞虱取食后植株功能损失系数(FPLI)及利用刺吸电位技术(EPG)研究褐飞虱的取食行为。【结果】 外施10 mmol∙L-1和50 mmol∙L-1浓度的海藻糖后,POD活性和可溶性糖含量显著上升,MDA含量显著下降,并且显著提高了水稻植株的功能损失指数,EPG结果发现海藻糖处理明显增加了N4波的持续时间。【结论】 适量的海藻糖改善水稻抗非生物胁迫的能力,但并不有利于对褐飞虱抗性的提高。
英文摘要:
[Objectives] Trehalose is involved in the response and
adaptation of plants to stress. This article aims to clarify the effect of
trehalose on the physiological and biochemical characteristics of rice and
resistance to the brown planthopper (BPH), Nilaparvata
lugens (Stål) (Hemiptera: Delphacidae), which will help to fully explore
the potential role of trehalose on rice and provide reference and basis for
subsequent research. [Methods] The effects of trehalose on the physiological
and biochemical characteristics of rice, including its resistance to the brown
planthopper were investigated after treatment with 10 mmol∙L-1 and 50 mmol∙L-1 trehalose;
specifically, the activity of the enzymes superoxide dismutase (SOD),
peroxidase (POD) and malondialdehyde (MDA), and soluble sugar content. In
addition, the functional plant loss index (FPLI) after BPH had fed on rice
plants and the effects of trehalose treatment on BPH feeding behavior were investigated
with an electrical penetration graph (EPG). [Results] The results showed that after applying
trehalose at a concentration of 10 mmol·L-1 and 50 mmol·L-1, the POD activity and soluble sugar content increased significantly,
the MDA content decreased significantly, and the function loss index of rice
plants was significantly improved. EPG results found that trehalose treatment
significantly increased the duration of the N4 wave. [Conclusion] Although appropriate levels of trehalose can
improve the resistance of rice to abiotic stress, it does not improve the
resistance of rice to BPH.