【目的】 本研究通过克隆苹果蠹蛾Cydia pomonella CPR (CpCPR) 基因cDNA序列，并对该基因的表达模式进行分析，为深入研究P450酶系在苹果蠹蛾对植物次生物质和杀虫剂解毒代谢过程中的作用提供理论基础。【方法】 以近缘昆虫的CPR氨基酸序列作为询问序列，在苹果蠹蛾转录组（SRX371333）中进行筛查比对，获得了苹果蠹蛾CPR（CpCPR）基因的cDNA序列。采用RT-PCR技术克隆目的基因的开放阅读框（ORF）。利用生物信息学软件分析目的基因的序列特征、3D结构和与其他昆虫CPR基因的系统进化关系。采用RT-qPCR技术测定CpCPR基因在苹果蠹蛾不同发育阶段（卵、幼虫、蛹和成虫）及幼虫不同组织部位（头部、表皮、脂肪体、中肠和马氏管）的表达水平。【结果】 克隆获得的苹果蠹蛾CpCPR基因的ORF为2 052 bp，编码683个氨基酸残基，预测的蛋白质分子量（Mw）为77.326 ku，理论等电点（pI）为5.65。CpCPR包含FMN区域、NADPH区域和FAD等昆虫CPR的典型特征。系统发育分析表明，苹果蠹蛾CpCPR与鳞翅目昆虫CPR基因聚在一枝。RT-qPCR结果表明，CpCPR基因在苹果蠹蛾的整个发育阶段均有表达，在幼虫期表达量最高；CpCPR基因在4龄幼虫的各部位均有表达，在中肠中的表达量最高。【结论】 克隆获得了苹果蠹蛾CpCPR基因的ORF序列，该基因在苹果蠹蛾主要取食阶段和消化器官中高表达，表明其可能在苹果蠹蛾对植物次生物质和杀虫剂解毒代谢过程中扮演重要作用。

[Objectives]  In order to improve understanding of the role of cytochrome P450 in the detoxification of plant secondary substances and insecticides, the CPR gene (CpCPR) of the codling moth, Cydia pomonella, was cloned and its expression profile was analyzed. [Methods]  The amino acid sequences of CPR genes from closely-related insect species were used as queries to search against the C. pomonella transcriptome database (SRX371333) and obtain the sequence of the C. pomonella CPR gene. RT-PCR was then used to amplify the Open Reading Frame (ORF) of the target gene. Bioinformatics software was used to compare the sequence characteristics, 3D structure and phylogenetic relationship of CpCPR to those of CPR from other insects. Real-time quantitative PCR (RT-qPCR) was used to determine the relative expression level of CpCPR in different developmental stages (eggs, 1-5 instar larvae, pupae, and adults) and in various tissues (head, cuticle, fat body, midgut, and Malpighian tubes). [Results]  The length of the CpCPR gene is 20 252 bp, which encodes 683 amino acids with a predicted molecular mass of 77.326 ku and a theoretical isoelectric point of 5.65. CpCPR contains typical insect CPR gene characteristics, such as an FMN region, an NADPH region and a FAD region. Phylogenetic analysis indicates that CpCPR clusters with the CPR gene of other Lepidoptera. RT-qPCR results show that the CpCPR gene is expressed in all developmental stages but is most abundant in the larval stage. In addition, CpCPR mRNA was detected in all tissues but was most abundant in the midgut in fourth-instar larvae. [Conclusion]  The ORF of the CpCPR gene was successfully cloned. This gene was most highly expressed during the larval stage and in the midgut of C. pomonella, indicating that it may play an important role in the detoxification of plant secondary substances and insecticides.