【目的】 获得广东省西方蜜蜂Apis mellifera所携带西奈湖病毒（Lake Sinai virus，LSV）基因组全长序列，并进行系统发育分析，确定其分类地位。【方法】 利用Sanger测序得到广东省西方蜜蜂所携带的LSV基因组全长，命名为LSV GIABR并上传至GenBank。从基因库中下载28个LSV基因组全长序列，以及34条RDRP序列，利用软件MEGA.X，分别使用最大似然法（Maximum Likelihood）和邻接法（Neighbor-Joining）构建的系统发育树，确立LSV GIABR的分类地位。另外，使用软件RDP4.0分析基因组是否发生重组及重组位置。【结果】 LSV GIABR基因组全长5 910 nt，包含ORF1（基因组位置78- 2615），RDRP（1852-3720），核衣壳蛋白Capsid（3739-5301）和ORF4（5346-5798）。全基因组和RDRP基因系统发育分析确定LSV GIABR属于LSV2，基因组重组分析推测其可能是美国和澳大利亚的LSV2株系的母本。【结论】 从广东省西方蜜蜂检测到的LSV基因组全长为5 910 nt，属于LSV2，并可能是美国和澳大利亚的LSV2株系的母本。

[Objectives]  To obtain the full-length genome sequence of the Lake Sinai virus (LSV) carried by Apis mellifera in Guangdong Province, and investigate LSV polymorphism with phylogenetic analysis. [Methods]  LSV GIABR was detected in Guangdong, China in 2016. The full-length genome of this virus was sequenced using Sanger sequencing and submitted to GenBank (accession number is MT732482). Twenty-eight genome sequences and 34 RDRP sequences were downloaded from GenBank, aligned and analyzed by MEGA.X using both the maximum likelihood (ML) and neighbor-joining (NJ) methods. A recombination event was predicted by RDP4.0. [Results]  The complete genome sequence contained 5 910 nucleotides, with an ORF1 segment (genome position from 78 to 2615) overlapping the RDRP segment (from 1852 to 3720), a capsid protein segment (from 3739 to 5301) and a hypothetical protein segment (from 5346 to 5798). Analysis of the genome lineage, RDRP gene and recombination events suggest that LSV GIABR belongs to SLV2 and is a putative, major, parental sequence of LSV2 isolates from the United States and Australia. [Conclusion]  LSV GIABR detected in A. mellifera in Guangdong, China belongs to the LSV lineage 2, and is predicted to be a major parental sequence of LSV2 isolates from the United States and Australia.