【目的】 蜜蜂触角作为嗅觉器官在蜜蜂的采集、繁衍等方面发挥着极其重要的作用。本研究以中华蜜蜂Apis cerana cerana（简称中蜂）采集蜂触角为材料，通过蛋白质全谱分析鉴定中蜂采集蜂触角所含蛋白质，为研究蜜蜂嗅觉感受机制奠定数据基础。【方法】 使用LC-MS/MS对中蜂采集蜂触角总蛋白进行鉴定；利用OmicsBean软件对中蜂采集蜂触角中鉴定的所有表达蛋白质进行GO分析和KEGG分析；采用qRT-PCR方法对不同级型中蜂触角中的NPC2（Niemann-Pick proteins of type C2）基因的转录情况进行检测。【结果】 LC-MS/MS鉴定结果显示，中蜂采集蜂触角中共鉴定到1 231个蛋白，其中包括14种与嗅觉相关的蛋白；GO分析与KEGG分析显示，分子功能分类中，绑定分子功能类别和催化活性功能类别所占比例最多。参与碳代谢、氨基酸、三羧酸循环、脂代谢等代谢通路的蛋白有297个，参与到环境信息加工和发育的有43个，参与到遗传信息加工的有67个，参与到小分子结合的约12%；qRT-PCR检测结果显示，NPC2在雄蜂的触角内表达量最高。【结论】 本研究所鉴定到的中蜂采集蜂触角蛋白质为触角的发育及其功能的发挥提供了物质基础与能量基础。此外，嗅觉相关蛋白的分析也为后续深入研究中蜂对气味物质的识别和转运机制提供了分子数据支撑。

[Objectives]  To investigate the role of the antennae in pollen collection and reproduction in bees and thereby provide a foundation for further study of the olfactory sensory mechanism of bees. [Methods]  Antennae were collected from Apis cerana cerana foragers and full spectrum protein analysis used to identify the antennal proteins. LC-MS/MS was used to identify the total antennal proteins and GO and KEGG analysis was performed on all proteins identified with OmicsBean. Semi-quantitative qRT-PCR was used to detect the transcription of NPC2 (Niemann-Pick proteins of type C2). [Results]  1 231 proteins were identified, including 14 olfactory-related proteins. GO and KEGG analyses indicate that most proteins are involved in binding and catalytic activity. 297 proteins are involved in metabolic pathways such as carbon and amino acid metabolism, TCA cycle and lipid metabolism. 43 proteins are involved in processing developmental and environmental information, and 67 in processing genetic information. About 12% of the proteins identified are involved in small molecule binding. qRT-PCR indicates that NPC2 was most highly expressed in the antennae of drones. [Conclusion]  The antennal proteins of foraging bees are mainly involved in antennal development and function. The results on the function of olfactory-related proteins provides support for further study of odorant recognition and the olfactory transport mechanism in A. cerana.