【目的】 通过对家蚕尿苷磷酸化酶1基因（BmUPase1）的克隆、原核表达、生物信息学分析及各组织和各时期的表达变化的研究，为揭示其在家蚕Bombyx mori中发挥的作用奠定基础。【方法】 以家蚕整蚕的cDNA为模板克隆获得BmUPase1基因的编码框序列。利用生物信息学软件分析序列特性。对该基因编码的蛋白质进行原核表达，为后续制备抗体提供条件。通过qPCR对BmUPase1在家蚕各组织中和各时期的表达量进行分析。【结果】 BmUPase1基因的完整ORF框序列全长1 188 bp，编码395个氨基酸，分子式为C₁₉₄₅H₃₀₇₄N₅₃₈0₅₉₁S₂₁，分子量大小为44.12 ku，具有尿苷磷酸化酶（UPase）超家族的保守结构域PNP_UDP_1。进化分析可知：家蚕与斜纹夜蛾Spodoptera litura、棉铃虫Helicoverpa armigera、小菜蛾Plutella xylostella、夏威夷红蛱蝶Vanessa tameamea等亲缘关系最近。qPCR结果显示BmUPase1基因在家蚕各个组织中都有表达，说明BmUPase1对于家蚕的生长发育具有重要的作用。时期表达谱结果显示，BmUPase1在家蚕化蛹、蛹期和蛾期的表达量升高，推测其与家蚕化蛹及化蛾有关。pET28a-BmUPase1重组载体在大肠杆菌BL21中成功表达，与预测大小一致。【结论】 本研究首次克隆了BmUPase1基因，其在家蚕的各个组织和各个时期都有表达，可能对家蚕的生长发育具有重要的作用。对其表达模式进行分析，为进一步研究蛋白质的功能奠定基础。

[Objectives]  To investigate the characteristics of uridine phosphorylase in the silkworm by cloning, prokaryotic expression, bioinformatics analysis, and thereby lay a foundation for elucidating the molecular mechanism of UPase in this economically important species. [Methods]  The CDS of BmUPase1 was cloned using Dazao cDNA and analyzed with bioinformatic software. The gene was then inserted into the prokaryotic expression vector pET28a to create a recombinant plasmid pET28a-BmUPase1, which was then transformed into Escherichia coli BL21 (DE3) to express BmUPase1. Real-time quantitative PCR was performed to profile the expression of the gene in different tissues and growth stages. [Results] Bioinformatics analysis showed that the BmUPase1 gene has an ORF of 1 188 bp and encodes 395 amino acids. BmUPase1 has one conserved domain, and its amino acid sequence identity is most similar to that of Spodoptera litura UPase (85.06 %). qPCR results indicate that the BmUPase1 gene is expressed in all tissues and growth stages, which suggests that it plays an important role in silkworm growth and development. The pET28a-BmUPase1 recombinant vector was successfully expressed in E. coli BL 21, and the expressed protein was of the predicted size. [Conclusion]  The silkworm Uridine phosphorylase gene can be successfully cloned and expressed. The results of this study lay a foundation for further study of the function of this gene in silkworm development.