【目的】 筛选近零磁场下粘虫 Mythimna separata雌蛾内稳定表达的内参基因，为在近零磁场下的粘虫靶标基因表达的定量分析准确性提供依据。【方法】 利用亥姆霍兹线圈产生近零磁场，分别在近零磁场（<500 nT）和地磁场（约50 μT）2种磁场强度下饲养粘虫。采用qRT-PCR技术测定2种磁场强度下饲养的粘虫初羽化雌蛾10种内参基因：β-肌动蛋白（β-Actin）、β-微管蛋白（β-TUB）、TATA盒结合蛋白（TBP）、延伸因子（EF-1α）、甘油醛-3-磷酸脱氢酶（GAPDH）、18S核糖体 RNA（18S rRNA）、28S核糖体 RNA（28S rRNA）、cGMP依赖性蛋白激酶（PKG）、核糖体蛋白L12（RPL12）和腺苷三磷酸酶（ATPase）的定量表达，利用geNorm、NormFinder、BestKeeper 以及在线综合分析系统RefFinder内参筛选分析软件分别对2种磁场强度下粘虫的内参基因稳定性进行评估筛选。【结果】 β-Actin和18S rRNA的稳定性在NormFinder结果中居于前两位，geNorm和BestKeeper结果中β-Actin和PKG基因稳定性最好，RefFinder 综合分析表明，β-Actin稳定性最好，其次为18S rRNA和PKG基因，β-TUB和TBP的表达稳定性在三个软件分析结果中都较差。geNorm软件进一步对内参基因的分析表明引入β-Actin和18S rRNA两个内参基因最佳。【结论】 明确了近零磁场强度下适用于粘虫初羽化雌成虫稳定表达的内参基因，确保了近零磁场下粘虫靶标基因转录表达水平的准确测定，为粘虫磁感受分子机制研究提供了重要工具。

[Objectives]  To identify a stable reference gene in adult Mythimna separata under a near-zero magnetic field in order to provide a way of assessing the accuracy of quantitative analysis of target gene expression in this species. [Methods] M. separata were raised under a near-zero magnetic field generated by a Helmholtz coil, with a control group raised under a geomagnetic field. 10 reference genes were chosen and evaluated，including β-actin (β-Actin), β-tubulin (β-TUB), TATA box binding protein (TBP), elongation factor (EF-1α), olealdehyde- 3-phosphate dehydrogenase (GAPDH), 18S RNA ribosome (18S rRNA), 28S ribosome RNA (28S rRNA), cGMP-dependent protein kinase (PKG), ribosomal protein L12 (RPL12) and adenosine triphosphatase (ATPase). RT-PCR technology was used to determine the expression level of these genes in newly emerged females raised under either the near-zero magnetic, or geomagnetic, fields, and four reference screening analysis software packages, geNorm, NormFinder, BestKeeper and RefFinder, were used to evaluate the stability of reference genes in each group. [Results]  β-Actin and 18S were ranked the most stable by NormFinder, and β-Actin and PKG by geNorm and BestKeeper. RefFinder analysis indicates that the most stable internal reference genes under the two different magnetic field treatments were the β-Actin, 18S and the PKG, genes. β-TUB and TBP were the most unstable expression reference genes. Further analysis using geNorm software indicates that it is best to use two reference genes. [Conclusion]  The most stable reference genes for adult female M. separata under different magnetic field intensities were identified, providing a foundation for the accurate quantification of the transcription of key genes involved in the magnetic reception mechanism in this species.