泽兰实蝇雌雄成虫转录组及差异分析
Transcriptome and differential analysis of adult male and female Procecidochares utilis Stone
高 平；李丽芳；兰明先；李梦月；廖贤斌；吴国星；高 熹
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DOI：10.7679/j.issn.2095-1353.2021.030
作者单位：云南农业大学植物保护学院，昆明 650201；云南农业大学植物保护学院，昆明 650201；云南农业大学植物保护学院，昆明 650201；云南农业大学植物保护学院，昆明 650201；云南农业大学植物保护学院，昆明 650201；云南农业大学植物保护学院，昆明 650201；云南农业大学植物保护学院，昆明 650201
中文关键词：泽兰实蝇；转录组；差异表达基因
英文关键词：Procecidochares utilis Stone; transcriptome; differentially expressed gene
中文摘要：
【目的】 泽兰实蝇Procecidochares utilis Stone是入侵杂草紫茎泽兰Eupatorium adenophorum Spreng的重要的专食性天敌，已成为控制紫茎泽兰的重要因子。本研究旨在获得泽兰实蝇转录组，并深入研究泽兰实蝇雌雄成虫差异表达基因。【方法】 利用Illumina高通量测序技术对泽兰实蝇雌雄成虫进行转录组测序和生物信息分析。【结果】 总共获得29 147条unigenes，平均长度为457 bp，同时将所得序列注释到七大数据库进行比对，共获得19 384条unigenes注释结果。分析发现11 331条差异表达基因；与雄成虫相比，雌成虫有2 640条unigenes表达量上调，8 691条unigenes表达量下调。【结论】 本研究获得了泽兰实蝇转录组，为今后泽兰实蝇性别相关研究提供了序列支持。
英文摘要：
[Objectives] Procecidochares utilis Stone is an important monophagous herbivore of the invasive weed Eupatorium adenophorum Spreng, and has become an important biological control of this pest plant. The purpose of this study was to obtain the P. utilis transcriptome, and investigate the molecular mechanism underlying differences between adult males and females of this species. [Methods] Illumina high-throughput sequencing technology was used to obtain transcriptome sequencing and conduct biological information analysis of P. utilis. [Results] A total of 29 147 unigenes with an average length of 457 bp were obtained. Gene sequences were annotated using seven databases, resulting in the identification of 19 384 unigenes. There were 11 331 differentially expressed genes; compared to adult males, adult females had 2 640 up-regulated, and 8 691 down-regulated, unigenes. [Conclusion] The transcriptome of P. utilis was obtained, which provides sequence data to support future research on the sexes of this species.