【目的】 玉米叶蝉缨翅缨小蜂Anagrus dmitrievi Triapitsyn & Hu 是玉米三点斑叶蝉Zyginidia eremita Zachvatkin卵期的重要寄生性天敌，在玉米三点斑叶蝉生物防治中具有广阔的应用前景。由于该寄生蜂个体微小，通过传统昆虫分类方法难以将其与同类卵寄生蜂准确分类。因此，本研究旨在为玉米叶蝉缨翅缨小蜂的快速准确鉴定提供技术支持。【方法】 在室内，采集玉米三点斑叶蝉卵饲养玉米叶蝉缨翅缨小蜂至成虫。根据玉米叶蝉缨翅缨小蜂成虫的体色、触角、翅等形态特征对标本进行鉴定，并利用无损伤法提取样本基因组DNA，通过PCR扩增和测序对COⅠ和ITS2基因进行DNA条形码分子标记试验。【结果】 形态学鉴定结果表明饲养获得的寄生蜂标本为玉米叶蝉缨翅缨小蜂。获得了该寄生蜂COⅠ和ITS2的部分序列，相似比对、遗传距离和分子进化树结果表明，玉米叶蝉缨翅缨小蜂COⅠ和ITS2序列分别与NCBI数据库中的同属其他种的相关基因有较高相似性，但存在一定遗传差异，可明显区分。【结论】 获得的玉米缨翅缨小蜂的COⅠ和ITS2基因序列可作为玉米叶蝉缨翅缨小蜂的DNA条形码，为后续该寄生蜂的快速准确鉴定提供了分子数据。

[Objectives]  To provide a convenient and efficient way of identifying Anagrus dmitrievi Triapitsyn & Hu, a small parasitic wasp with potential as a biological control for the corn pest Zyginidia eremita Zachvatkin (Hemiptera, Cicadellidae, Typhlocybinae), that is difficult to identify on the basis of morphological features. [Methods]  Eggs of Z. eremita were collected and hatched in a laboratory to obtain adult A. dmitrievi which were then identified according to morphological characters such as body color, antennae and wings. Genomic DNA was extracted from the adult wasps using a non-damaging DNA extraction method, and DNA barcoding tests performed on the COⅠ and ITS2 genes after PCR amplification and sequencing. [Results]  Multiple sequence alignments, genetic distance and phylogenetic analyses indicate that although A. dmitrievi COⅠ and ITS2 are highly homologous to those of other Anagrus species listed in the NCBI databases, there are some clearly distinguishable differences. [Conclusion]  COⅠ and ITS2 gene sequences were obtained from male and female A. dmitrievi are sufficiently distinctive to provide a foundation for the development of a molecular rapid identification system for this small, parasitic wasp.