基于代谢组方法筛选蜜蜂副伤寒相关差异代谢物的研究
Using metabolomic methods to screen metabolites associated with paratyphoid disease in honeybees
龙登隆 牛庆生 杜亚丽 刘玉玲 王 志 常志光 徐 凯
点击：861次 下载：31次
DOI：10.7679/j.issn.2095-1353.2021.061
作者单位：吉林省养蜂科学研究所，吉林 132108
中文关键词：蜜蜂；越冬期；副伤寒；代谢组学；差异代谢物
英文关键词：honeybee; overwinter stages; paratyphoid disease; metabolomics methods; differential metabolites
中文摘要：
【目的】 蜜蜂副伤寒是西方蜜蜂Apis mellifera越冬期的主要易感疾病。本研究旨在探究蜜蜂副伤寒疾病相关的代谢物和代谢通路。【方法】 以越冬末期的西方蜜蜂为研究对象，利用基于高效液相质谱联用的非靶向代谢组学方法检测副伤寒患病群和正常越冬群工蜂肠道中代谢物的变化。【结果】 蜜蜂副伤寒患病个体和正常蜜蜂个体在正、负离子模式下分别获得626个和518个差异代谢物，在正、负离子模式下最显著差异的10种代谢物中筛选到镰刀菌氧萘满酮、奎尼丁、褪黑素、去氧紫草素等与蜜蜂物质代谢障碍、细胞凋亡和抗氧化应激相关的差异代谢物。此外，差异代谢物显著富集于与氨基酸代谢密切相关的氨酰-tRNA合成、蛋白质消化吸收、谷胱甘肽代谢、矿物质代谢、多种氨基酸代谢等7个代谢通路。【结论】 蜜蜂患副伤寒后肠道中代谢物水平发生显著变化，其中氨基酸和蛋白质的代谢异常可能是蜜蜂副伤寒发病的主要原因。
英文摘要：
[Objectives] To investigate metabolites and metabolic pathways related to paratyphoid disease in the Western honeybee. [Methods] Changes in metabolites in the guts of bees from diseased and healthy colonies at the end of the overwintering stage were detected using a non-targeted metabolomics method based on liquid chromatography mass spectrometry. [Results] 626 and 518 different metabolites in the positive and negative ion mode, respectively, were detected in diseased and healthy colonies. Many metabolites related to substance metabolism disorder, apoptosis and antioxidant stress, such as fusarochromanone, quinidine, melatonin and deoxyshikonin, were identified in the ten most significant differences among the positive and negative ion modes. In addition, differential metabolites were significantly enriched in 7 pathways related to amino acid metabolism, including aminoacyl tRNA synthesis, protein digestion and absorption, glutathione metabolism, mineral metabolism and amino acid metabolism. [Conclusion] Metabolite levels in the guts of honeybees are significantly altered by paratyphoid disease and metabolic abnormalities in amino acids and proteins could be the main cause of paratyphoid disease symptoms.