RNAi递送系统在害虫防治中的研究进展
Progress in research on the use of RNAi delivery systems in pest control
肖 蓓 幸诗斯 邓 丹 彭 威 翟宗昭
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DOI：10.7679/j.issn.2095-1353.2021.077
作者单位：动物肠道功能调控湖南省重点实验室，淡水鱼类发育生物学国家重点实验室，湖南省动物肠道生态与 健康国际科技创新合作基地，湖南师范大学，长沙 410081；动物肠道功能调控湖南省重点实验室，淡水鱼类发育生物学国家重点实验室，湖南省动物肠道生态与 健康国际科技创新合作基地，湖南师范大学，长沙 410081
中文关键词：RNA干扰；喂食；表皮渗透；微生物；纳米粒子；害虫防治
英文关键词：RNA interference; ingestion; cuticular penetration; microbes; nanoparticles; pest control
中文摘要：
RNAi是由dsRNA引发的，靶向目的基因的高效与特异的基因沉默技术。由于其高效性、特异性和便捷性，RNAi技术广泛应用于基因功能研究、高通量目的基因筛选、基因治疗、药物靶标预测和农业病虫害防治等领域。考虑到RNAi效率、安全性和预期靶基因下调的潜在障碍，RNAi提供高效防治应用的前提是以合适的方式递送效应RNA。本文对近期农业重要性害虫和病媒害虫防治应用中开发的RNAi递送系统进行综述，对这些RNAi递送系统的干扰效率和多重进入位点作了比较，并展望了RNAi递送系统在害虫防治中的应用，以期更好地完善RNAi技术在昆虫学研究和害虫防治中的应用。
英文摘要：
RNAi is triggered by
dsRNA and causes an efficient and specific cleavage of the target gene. Because
of its high efficiency, specificity and convenience, RNAi is widely used in
gene function studies, high throughput screening of target genes, gene therapy,
drug target prediction and agricultural pest control. RNAi can offer
unprecedented levels of control, but an appropriate approach, giving due
consideration to potential barriers to RNAi efficiency, safety and the intended
purpose of the knockdown, is fundamental to its successful deployment. We
review and compare the efficiency of recent innovations in RNAi delivery,
including the different delivery systems used in agricultural pest and vector
pest control. We discuss future applications of RNAi delivery systems in pest
control in order to promote
advances in entomological research based on better RNAi technologies.