柑橘大实蝇信息素鉴定及EAG测试
Electroantennographical response of Bactrocera minax to pheromones
李 可 李 凡 陈 楠 XIA Yu-Lu 樊永亮 刘同先
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DOI：10.7679/j.issn.2095-1353.2021.089
作者单位：西北农林科技大学旱区作物逆境生物学国家重点实验室，农业部西北黄土高原作物有害生物综合治理重点实验室， 杨凌 712100；华南师范大学生命科学学院，广东省昆虫发育生物学与应用技术重点实验室，广州 510631； North Carolina State University-Center for Integrated Pest Management, Raleigh 27695, USA
中文关键词：柑橘大实蝇；信息素；触角电位；N-(3-甲基丁基)乙酰胺；挥发物
英文关键词：Bactrocera minax; pheromone; electroantennogram; N - (3-methyl-butyl) acetamide; volatile
中文摘要：
【目的】 分析和鉴定柑橘大实蝇Bactocera (Tetradacus) minax (Enderlein)信息素。【方法】 采用顶空固相微萃取方法（HS-SPME）收集不同时间段柑橘大实蝇成虫挥发物，进一步结合气相色谱质谱联用技术（GC-MS）鉴定分析和触角电位技术（EAG）进行行为测试。【结果】 N-(3-甲基丁基)乙酰胺为柑橘大实蝇成虫合成的一种挥发性化合物，且雌雄成虫都可以释放该化合物。触角电位技术测定不同日龄柑橘大实蝇成虫对1、10、100和1 000 μg 4种不同剂量N-(3-甲基丁基)乙酰胺的触角电位反应结果表明，柑橘大实蝇成虫对N-(3-甲基丁基)乙酰胺具有显著的触角电位反应，触角电位反应最适含量范围为大于100 μg，剂量以及剂量×性别会导致柑橘大实蝇显著的触角电位反应差异。【结论】 N-(3-甲基丁基)乙酰胺可能为柑橘大实蝇信息素，这一结果可为柑橘大实蝇化学通讯研究和生物防治应用提供一定的理论基础。
英文摘要：
[Objectives] To analyze and identify the pheromones of Bactocera (Tetradacus) minax (Enderlein). [Methods] Volatiles from male and female adults were
collected at various times by Headspace-Solid Phase Micro Extraction (HS- SPME), and identified
chemically by Gas Chromatography-Mass Spectrometry (GC-MS) and behaviorally by
Electroantennography (EAG). [Results] GC-MS identified N-(3-methyl-butyl)
acetamide as a volatile released by both adult male and female B. minax.
The EAG responses of 3-day-old and 15-day-old B. minax to 1, 10, 100 and 1 000 μg of N-(3-methyl-butyl) acetamide
were measured. Adults had a strong EAG response to N-(3-methyl-butyl)
acetamide. The optimum concentration for EAG was from 100 to 1 000 μg. EAG
response varied with dose and dose×sex. [Conclusion] N-(3-methyl-butyl) acetamide could be a
pheromone of B. minax. The results
provide information useful for further study of chemical communication in B. minax and the biological control of
this pest.