
温湿度对金蝉虫卵保存的影响
Effects of temperature and humidity on the preservation of Cryptotympana atrata eggs
曹 烨 邓 盼 孙德永 唐楚飞 魏兰君 廖怀建 孙洪武
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DOI:10.7679/j.issn.2095-1353.2021.140
作者单位:江苏省农业科学院休闲农业研究所,南京 210014;徐州君晓生态农业科技有限公司,徐州 221732
中文关键词:金蝉;虫卵保存;温度;湿度;存活;孵化
英文关键词:Cryptotympana atrata; egg preservation; temperature; humidity; survival; incubation
中文摘要:
【目的】 为提升金蝉Cryptotympana atrata虫卵孵化质量,提高金蝉养殖经济效益,本研究对金蝉虫卵保存的适宜温湿度条件进行研究。【方法】 通过正交实验设计,设置温度(10、15、20、25和30 ℃)和湿度(35%、45%、55%、65%和75%)梯度,测定金蝉虫卵在不同温湿度条件下保存60 d 的生物学参数,包括虫卵干瘪程度、虫卵剩余量、虫卵孵化时间及虫卵孵化率。【结果】 在温度为10 ℃和湿度为35%-75%的条件下金蝉虫卵保存效果最佳,保存60
d后,虫卵孵化率可达73%以上,虫卵横截面厚度可达374.23-400.00
μm。不同温湿度条件下,金蝉虫卵干瘪程度不同。温度为10
℃和湿度为35%-75%的条件下,干瘪程度最低;温度为15-20
℃和湿度为45%-75%下保存次之;25-30
℃和湿度65%-75%下保存干瘪程度最高。金蝉虫卵于10-15
℃和湿度35%-75%条件下剩余量最多;20
℃和湿度45%-75%下保存次之;25
℃和湿度75%下保存再次之;30
℃下保存的虫卵剩余量最少,35%湿度下保存60
d,虫卵全部干瘪死亡。同一保存湿度下,金蝉虫卵孵化时间随保存温度升高显著缩短,金蝉虫卵孵化率随保存温度升高而显著降低;温度10-20
℃时,不同湿度条件下保存的虫卵,孵化率无显著差异;温度25-30
℃时,虫卵孵化率随保存湿度升高而显著提升。【结论】 温度和湿度均能显著影响金蝉虫卵的保存效果。温度为10
℃和湿度为35%-75%是金蝉虫卵保存的最佳温湿度条件,且保存温度越低,虫卵对湿度的要求就越低;保存温度为15-20
℃时,湿度越高,虫卵保存效果越好;当温度达到25
℃时,虫卵保存湿度需在65%以上;当温度达到30
℃时,虫卵保存湿度需在75%以上。
英文摘要:
[Objectives] To improve the hatching rate of Cryptotympana atrata eggs and increase
the cost effectiveness of breeding this species in captivity. [Methods] An orthogonal experimental design was used to
determine the effects of different temperatures (10, 15, 20, 25 and 30 ℃) and humidity (35%, 45%, 55%, 65% and 75%) on
the biological parameters of C. atrata eggs
over a 60 day period, including the degree of shriveling, survival, incubation
period and hatching rate. [Results]
A temperature of 10 ℃ and humidity of 35%-75% were best; under these conditions the hatching rate was
>73% and the average cross sectional thickness of eggs was up to
374.23-400.00 μm. Eggs underwent different degrees of shriveling under the
different temperature and humidity conditions. The least shriveling occurred at
a temperature of 10 ℃ and a humidity of 35%-75%. A greater amount of
shriveling was observed at temperatures of 15-20 ℃ and humidity of 45%-75%, but the most
shriveling was observed at temperatures of 25-30 ℃ and humidity of 65%-75%. The number of
surviving eggs was highest at temperatures of 10-15 ℃ and humidity of 35%-75%. Fewer eggs survived
after storage at 20 ℃ and 45%-75% humidity, and
even fewer when stored at 25 ℃ and 75% humidity. The least
number of eggs survived after storage at 30 ℃; all eggs stored at
30 ℃ and 35% humidity withered and died after 60
days. At the same humidity, the incubation time and the hatching rate of eggs
decreased significantly at higher storage temperatures. There was no
significant difference in the hatching rate of eggs preserved under various
humidity conditions at temperatures between 10-20 ℃, but the hatching rate of eggs increased
significantly with increasing humidity in the 25-30 ℃ range. [Conclusion] Both temperature and humidity significantly
affected the success of preserving C.
atrata eggs. 10 ℃ and humidity
35%-75% were the best conditions for preserving the eggs of this species. Lower temperatures
reduce the humidity required for successful egg preservation. At temperatures
of 15-20 ℃, the higher the humidity,
the more eggs survived. Humidity of >65% was required to preserve eggs at 25
℃, and >75% humidity was required to
preserve eggs at 30 ℃.