一种使用Image J重构昆虫感器支配神经的方法
A method of reconstructing innervating nerves of insect sensilla using Image J
程振丰；付丙鲜；祝增荣；洪 健
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DOI：10.7679/j.issn.2095-1353.2022.024
作者单位：浙江农林大学现代农学院，临安 311300；浙江大学农业与生物技术学院昆虫科学研究所，杭州 310058； 浙江大学农生环测试中心，杭州 310058
中文关键词：Image J软件；昆虫；感器；支配神经；三维重构
英文关键词：Image J; insect; sensilla; innervating nerves; three-dimensional reconstruction
中文摘要：【目的】 Image J是一款基于Java的完全开源软件，本文通过Image J重构昆虫感器支配神经，以期提供一种开源的三维重构方法。【方法】 Image J重构包括调整系列图片亮度及对比度、图像校准、手动绘制填色图片、导入填色图片、通过图形工作站运行3D Viewer。【结果】 基于Image J，完成了灰飞虱触角盘状感器支配神经的三维重构，重构模型生动展示了盘状感器支配神经元组的数量及空间延展关系。在三维查看器中可旋转、放大三维模型观察精细结构，也可调整阈值观察特定结构，还可调整模型透明度及模型整体颜色。【结论】 Image J具有广阔的升级空间和应用前景，有望为昆虫、其他节肢动物及生物组织的三维重构提供有力支持。
英文摘要：
[Objectives] Image J is a fully open-source Java-based software
package. We used Image J to reconstruct the dendritic innervations of insect
sensilla, thereby providing an open source, three-dimensional reconstruction
method. [Methods] We followed the following steps; adjust the
brightness and contrast of serial pictures, calibrate images, manually add
color and import pictures, and finally, run the 3D viewer through a graphics
workstation. [Results] We successfully completed 3D reconstruction
of the innervating nerves of sensilla placodea on antennae of the small brown
planthopper. The reconstructed model vividly demonstrates the number of
neuronal groups of sensilla placodea and their spatial relationships with each
other. The 3D viewer allowed images to be rotated and enlarged to observe fine
structures, adjustment of the threshold value to observe the specific
structures and adjustment of the transparency and overall color of the model. [Conclusion] Image J has great potential for application
in this field and is expected to provide technical support for the 3D
reconstruction of the morphology of insects, other arthropods and biological
tissues.