茶核?苏云菌悬浮剂对灰茶尺蠖的 田间防控应用
The application of Ectropis obliqua nucleopolyhedroviruses preparations as a biological control for Ectropis grisescens in the field
唐美君 郭华伟 李 红 张欣欣 王志博 姚惠明 周孝贵 肖 强
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DOI：10.7679/j.issn.2095-1353.2022.032
作者单位：中国农业科学院茶叶研究所，杭州 310008；农业部茶叶质量安全控制重点实验室，杭州 310008； 中国农业科学院研究生院，北京100081
中文关键词：核型多角体病毒；灰茶尺蠖；应用
英文关键词：nucleopolyhedrovirus; Ectropis grisescens; application technology
中文摘要：
【目的】 茶尺蠖核型多角体病毒EcobNPV是一种重要的昆虫病原微生物，可侵染茶尺蠖Ectropis obliqua和灰茶尺蠖Ectropis grisescens 2种宿主。为探明茶尺蠖核型多角体病毒制剂的田间应用技术，对影响防效的3个因子喷施方式、剂量和虫龄进行研究。【方法】 采用植保无人机和人工喷施2种喷施方式，比较茶核·苏云菌悬浮剂（1×104 PIB·2 000 IU/μL）防治灰茶尺蠖的效果；设置75、150和375 mL/667 m2 3种剂量，比较茶核·苏云菌悬浮剂对灰茶尺蠖的防治效果；并比较其对灰茶尺蠖1龄、2龄和3龄不同虫龄的防治效果。【结果】 在相同剂量下，用植保无人机和人工喷施病毒制剂对灰茶尺蠖的总防效分别为69.0%和67.6%，两者无显著差异；在75、150和375 mL/667 m2 3种剂量下病毒制剂对灰茶尺蠖的防效差异显著，其中375 mL/667 m2剂量下的防效最高，150 mL/667 m2其次，150 mL/667 m2和375 mL/667 m2剂量对灰茶尺蠖的总防效均在90%以上；病毒制剂对灰茶尺蠖1龄、2龄和3龄幼虫的防效分别为93.3%、78.7%和67.8%，对1龄幼虫的防效显著高于3龄。【结论】 茶核·苏云菌悬浮剂防治灰茶尺蠖可选用植保无人机喷施，剂量可选用150-375 mL/667 m2，以1龄幼虫期喷施防效为最佳。
英文摘要：
[Objectives] To investigate techniques for using Ectropis obliqua nucleopolyhedrovirus (EcobNPV) preparations, a pathogen that can infect both Ectropis obliqua and Ectropis grisescens, to control these pests. [Methods] The effects of different application methods (drone and sprayer）, larval instar (1st, 2nd and 3rd instar larvae) and dosages (75, 150 and 375 mL/667 m2) on the effectiveness of EcobNPV preparations containing both 1×104 PIB/μL EcobNPV and 2 000 IU/μLBacillus thuringensis, were tested in field trials. [Results] There was no significant difference in the effectiveness of EcobNPV preparations of the same dosage applied with either a drone (69.0%) or sprayer (67.6%). The optimal dose of EcobNPV preparations was 375 mL/667 m2, followed by 150 mL/667 m2; the effectiveness of both doses exceeded 90%. The effectiveness of EcobNPV preparations on 1st, 2nd and 3rd instar larvae was 93.3%, 78.7% and 67.8%, respectively, and its effectiveness on 1st instar larvae was significantly higher than that on 3rd instar larvae. [Conclusion] EcobNPV preparations can be applied by drones to control tea geometrids. First instar larvae are most susceptible to EcobNPV preparations and the optimal dose is 150-375 mL/667 m2.