
内唇在蜉金龟亚科中用于分属和分族的分类学价值
The taxonomic value of the epipharynx in the Aphodiinae
杜萍萍 佟一杰 路园园 李晓璇 李露露 白 明
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DOI:10.7679/j.issn.2095-1353.2022.039
作者单位:安徽大学物质科学与信息技术研究院,合肥 230601;中国科学院动物研究所动物进化与系统学重点实验室,北京 100101; 新疆大学生命科学与技术学院,乌鲁木齐 830046;安徽师范大学生命科学学院,芜湖 241000; 中国科学院大学, 北京 100049;海南省崖州湾种子实验室,三亚 572024
中文关键词:高级阶元;进化;形态差异;系统发育;几何形态学
英文关键词:high-level taxon; evolution; morphological difference; phylogeny; geometric morphometrics
中文摘要:
【目的】 蜉金龟亚科(鞘翅目:金龟科)成虫外部形态高度趋同,种间相似性大,属族等高级阶元的鉴别特征混乱,因此构建新的形态指标系统极为重要。近年来内唇形态广泛应用于蜉金龟的分类学研究,并能有效区分部分种类,然而内唇在高级阶元的分类学价值和应用范围仍未可知。【方法】 本文选取276属818种金龟为研究对象,其中包括蜉金龟亚科7族259属799种,以及外群样本17属19种(蜣螂亚科13属14种,沙金龟亚科4属5种),利用几何形态学手段量化蜉金龟内唇的形态学信息,评估了属级和族级的区分度,并联合支序系统学方法,重建了蜉金龟祖先内唇形态。【结果】 根据主成分分析显示,属级阶元的形态总方差为0.032 131 27,族级为0.032 914 63,内唇在属族间变异程度接近(两者相差约3%),揭示了内唇形态在高级阶元间基本保持稳定,适合用于分类鉴定。为了进一步探索内唇在高级阶元的分类归属是否也接近,随之对样本进行判别函数分析。结果显示,仅基于内唇形态,98.49%的属可被正确鉴定,族级有98.41%的正确率,表明内唇在属级阶元间的判别归类略胜于族级阶元,内唇适合应用于高级阶元的判别归类。重建的内唇祖先形态显示其多变区域包括上内唇根基部、侧缘,内唇前区和内唇外缘等6个。【结论】 本研究利用定量形态学方法证实了内唇适用于蜉金龟高级阶元分类,同时为外部形态趋同类群提供了新的特征筛选与评估范式。
英文摘要:
[Objectives] To construct a new morphological index system for the Aphodiinae
(Coleoptera: Scarabaeidae), the adults of which are very similar in external
morphology complicate accurate classification into genera and tribes. Previous
studies have shown that the morphology of the epipharynx can effectively
distinguish some species, but it was not known whether the same criteria could
be used to identify higher taxa. [Methods] 818 species belonging to 276 genera
were used as research samples, including 799 species, 259 genera and 7 tribes
of the Aphodiinae, and 19 species and 17 genera as outgroup samples (13 genera
and 14 species of the Scarabaeinae, 4 genera and 5 species of the Aegialiinae).
Morphological information on the epipharynx was quantified by means of
geometric morphometrics. The ability of this information to classify species to
the generic and tribal levels was then evaluated, and the morphology of the
ancestors of the Aphodiinae was reconstructed using cladistic methods based on
epipharyngeal morphology. [Results] Based on Principal Component Analysis, the
total morphological variation at the genus and at tribal levels was estimated
to be 0.032 131 27 and 0.03 291 463, respectively. The degree of epipharyngeal
variation among genera and tribes was small (about 3%), which reveals that
epipharyngeal morphology has remained basically stable in the higher-level
taxa. In order to determine whether the epipharynx is more similar among higher
taxa, we also performed a discriminant function analysis. The results showed
that 98.49% of genera and 98.41% of tribes can
be correctly identified on the basis of epipharyngeal differences, which
suggests that, although epipharyngeal differences
are slightly better at discriminating genera than tribes, they are generally
suitable for the discrimination and classification of the higher taxa of the
Aphodiinae. Combined with a phylogenetic tree, the reconstructed ancestral
shape of the epipharynx suggests that it has six variable morphological
features, including the base, the lateral margin of the epitorma, the corypha
and the shape of the epipharyngeal marginal. [Conclusion] The results
validate the previous use of some epipharyngeal features to resolve the
taxonomy of the Aphodiinae, and also has identified some new morphological
indexes. The epipharyngeal features are suitable for the classification of
Aphodiinae into genera and tribes using quantitative morphology, and this
approach has provided a new paradigm for resolving the taxonomy of groups with
a high degree of external morphological convergence.