【目的】 克隆获得草地贪夜蛾Spodoptera frugiperda V-ATP酶（Vacuolar-type proton，ATPase）V₁结构域A、B、C和D亚基的cDNA序列，分析其在草地贪夜蛾不同生长阶段的表达情况，为筛选新型农药的靶点奠定基础。【方法】 利用PCR技术获得草地贪夜蛾V-ATP酶V₁结构域A、B、C和D亚基基因的ORF区域，采用qRT-PCR技术检测各基因在草地贪夜蛾不同发育时期的表达水平。【结果】 A、B、C和D亚基基因均在不同鳞翅目昆虫中高度保守。A-D亚基在草地贪夜蛾各生长阶段均有表达，在幼虫阶段和成虫阶段的表达量均较高，表明V-ATP酶可能影响幼虫生长发育和成虫交配繁殖。卵期和蛹期表达量低，这与卵期和蛹期昆虫各项生命活动低有关。各亚基在各发育时期的表达量存在差异，说明V-ATP酶A、B、C和D发挥不同的功能。【结论】 从草地贪夜蛾中克隆了V-ATP酶V₁结构域A、B、C和D亚基4个基因在草地贪夜蛾不同发育阶段表达量具有差异，推测其对草地贪夜蛾的生长、发育和繁殖具有重要的调控作用。

[Objectives]  To clone the cDNA sequences of the A, B, C and D subunit genes of the Spodoptera frugiperda V-ATPase V₁ region and compare the expression of these genes in different developmental stages in order to both improve the prevention and control of S. frugiperda and identify new target genes for pesticides. [Methods]  The sequences of the genes encoding the V-ATPase subunits A, B, C and D were cloned with PCR (polymerase chain reaction), and the expression of each gene was analyzed in different development stages with real-time quantitative PCR. [Results]  The A, B, C and D subunit genes of the V₁ region are highly conserved in the Lepidoptera. All four genes were expressed during the entire developmental process but were more highly expressed in the larval and adult stages, suggesting that V-ATPase plays an important role in the growth and development of larvae and in adult reproduction. The expression of the four subunit genes differed in different development stages, suggesting that each has different functions. [Conclusion]  The four V-ATPase V₁ subunit A, B, C and D genes had different expression levels in different developmental stages of S. frugiperda, which suggests that they play an important role in regulating the growth, development and reproduction of this species.