【目的】 田间草地贪夜蛾Spodoptera frugiperda（J.E. Smith）蛹与斜纹夜蛾Spodoptera litura Fabricius、甜菜夜蛾Spodoptera exigua（Hübner）、粘虫Mythimna separata（Walker）、棉铃虫Helicoverpa armigera（Hübner）和亚洲玉米螟Ostrinia furnacalis 5种重要鳞翅目害虫蛹容易混淆。明确草地贪夜蛾与其它5种重要的鳞翅目害虫蛹的形态差异，为田间鳞翅目害虫蛹的准确鉴别和田间调查提供依据。【方法】 采用体式显微镜拍照观察并分析草地贪夜蛾蛹、斜纹夜蛾蛹、甜菜夜蛾蛹、粘虫蛹、棉铃虫蛹和亚洲玉米螟蛹的外部形态特征，根据相关特征差异编制了区分草地贪夜蛾与此5种鳞翅目害虫的检索表和快速区分表。【结果】 草地贪夜蛾蛹主要特征为中足末端到达下颚末端之前，触角末端到达中足末端之前；中胸背板前缘略直；第4-7腹节背面前缘着生多行粗点刻；腹部末端臀棘上着生1对基部分开呈八字形的刺。草地贪夜蛾蛹腹部末端臀棘部位与其它5种害虫均不相同，辅以附肢位置、中胸背板前缘线和腹部点刻等特征可以准确将其与斜纹夜蛾、甜菜夜蛾、粘虫、棉铃虫和亚洲玉米螟的蛹区分。斜纹夜蛾中胸背板前缘线和臀棘上的刺与草地贪夜蛾有异；甜菜夜蛾下颚与中足位置及臀棘上的刺与草地贪夜蛾有异；粘虫腹部点刻和臀棘上的刺与草地贪夜蛾有异；棉铃虫触角末端位置和腹部末端的刺与草地贪夜蛾有异；亚洲玉米螟腹足遗迹和臀棘上的刺与草地贪夜蛾有异。【结论】 根据蛹的结构特征，可以区分草地贪夜蛾与斜纹夜蛾、甜菜夜蛾、粘虫、棉铃虫和亚洲玉米螟等害虫的蛹。

[Objectives]  The pupae of Spodoptera frugiferda are easily confused with those of other Lepidoperan pests, including S. litura, S. exigua, Mythimna separata, Helicoverpa armigera, and Ostrinia furnacalis. Documenting morphological differences between the pupae of these species will therefore facilitate the accurate identification of Lepidopteran pests in the field. [Methods]  The external morphological characteristics of S. frugiferda, S. litura, S. exigua, M. separata, H. armigera and O. furnacalis pupae were observed under a microscope, and a key table and rapid discrimination table compiled to distinguish those of S. frugiferda from those of the other species. [Results]  The main diagnostic characteristics of S. frugiferda pupae were the midleg extending beyond the end of the maxillae, the antenna reaching the end of the midleg, the slightly straight front edge of the mesonotum, multiple lines of thick dots on the dorsal anterior edge of the 4th-7th abdominal segment and a pair of terminal spines with a wide angle of separation at the tip of the abdomen. This difference between the terminal spines of S. frugiferda and those of S. litura, S. exigua, M. separata, H. armigera, and O. furnacalis is the main diagnostic feature distinguishing S. frugiferda from the other species. In addition, differences in the position of the external grooves (sutures) between the tips of appendages, the slightly straight front edge of mesonotum and the presence of abdominal spots are also diagnostic. S. litura differs from S. frugiferda in the shape of the front edge of mesonotum and the terminal spines on the tip of abdomen. S. exigua differs from S. frugiferda in the position of the maxillae and midleg and the terminal spines on the tip of abdomen. M. separata can be distinguished from S. frugiferda by the absence of abdominal spots and the terminal spines on the tip of abdomen. H. armigera differs from S. frugiferda in the position of the end of the antennae and the terminal spines on the tip of the abdomen. O. furnacalis differs from S. frugiferda in the structure of the abdominal legs and the terminal spines on the tip of the abdomen. [Conclusion]  The pupae of S. frugiferda, S.litura, S. exigua, M. separata, H. armigera, and O. furnacalis can be distinguished on the basis of morphological characteristics.