【目的】 明确草地贪夜蛾Spodoptera frugiperda幼虫体内消化酶对常用杀虫剂甲氨基阿维菌素苯甲酸盐（甲维盐）和氯虫苯甲酰胺的响应及亚致死浓度(LC₂₅)胁迫后子代生物学参数的变化，为杀虫剂的科学合理使用及害虫的综合防治提供理论依据。【方法】 在甲维盐和氯虫苯甲酰胺对草地贪夜蛾3龄幼虫毒力的基础上，分别用此2类杀虫剂亚致死浓度处理的小麦叶片饲喂3龄幼虫，比较取食48 h后F₀和F₁代幼虫体内胃蛋白酶(Pepsin)、α-淀粉酶(α-AMS)和脂肪酶(Lipase)的活性变化，分析亚致死浓度对子代发育历期、体重、产卵期、产卵量和种群参数的影响。【结果】 2类杀虫剂亚致死浓度胁迫下草地贪夜蛾F₀代幼虫胃蛋白酶活性显著低于对照(P＜0.05)，且甲维盐对F₁代幼虫胃蛋白酶活性具有持续的显著抑制作用，氯虫苯甲酰胺处理后其胃蛋白酶活性降低差异不显著。α-淀粉酶和脂肪酶活性与对照相比无显著差异。经2类杀虫剂亚致死浓度处理后，子代(F₁)高龄幼虫(6龄)和蛹体重显著降低，蛹期显著延长，产卵期和平均单雌产卵量显著减少。对F₁代种群参数的影响体现在，净增值率(R₀)、内禀增长率(r_m)和周限增长率(λ)显著降低，平均世代周期(T)和种群加倍时间(DT)延长。【结论】 草地贪夜蛾在甲维盐和氯虫苯甲酰胺亚致死浓度作用下，幼虫胃蛋白酶活性被抑制，子代发育历期延长，繁殖力降低，种群增长缓慢，2种杀虫剂亚致死浓度对草地贪夜蛾有持续控制作用。

[Objectives]  The objective To investigate the effects of two different insecticides on the digestive enzymes of Spodoptera frugiperda larvae, and assess changes in the biological characteristics of the offspring of S. frugiperda exposed to sublethal concentrations (LC₂₅) of these pesticides. [Methods]  Third instar S. frugiperda larvae were exposed to sublethal concentrations of emamectin benzoate and chlorantraniliprole, based on the virulence of each pesticide. These larvae were then fed on wheat leaves and the  pepsin (Pepsin), α-amylase (α-AMS), and lipase (LPS) activity levels of both the F₀ (parental) and F₁ (offspring) generations were measured and compared 48 hours after feeding. In addition, we analyzed the impact of exposure to sublethal pesticide concentrations on offspring developmental period, weight, oviposition characteristics, and population parameters. [Results]  Exposure to sublethal concentrations of both insecticides significantly reduced the pepsin activity of the F₀ generation compared to the control (P<0.05). Furthermore, exposure to emamectin benzoate consistently, and significantly, inhibited the pepsin activity of F₁ larvae. Exposure to chlorantraniliprole did not, however, significantly reduce pepsin activity. No significant differences were observed in α-amylase and lipase activity compared to the control group. Exposure to sublethal pesticide concentrations reduced the weight of F₁ 6th instar larvae and pupae, significantly prolonged the pupal period, decreased the oviposition period, and reduced average egg production per female. These effects resulted in a significant decrease in the F₁ generation’s net value-added rate (R₀), intrinsic growth rate (r_m), weekly growth rate (λ), and also extended its average generation period (T) and population doubling time (DT). [Conclusion]  Exposure to sublethal concentrations of emamectin benzoate and chlorantraniliprole, inhibited the pepsin activity of S. frugiperda larvae, and prolonged the developmental period, lowered the fecundity and slowed the population growth of the F₁ generation. Exposure to sublethal concentrations of these two insecticides can therefore provide sustained control of S. frugiperda.