固相微萃取技术在昆虫信息化学物质 提取中的应用进展
Application of solid phase microextraction to the extraction of insect semio-chemicals
苏钟琪，董 祥，谢雯其，马 涛，温秀军
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DOI：10.7679/j.issn.2095-1353.2023.049
作者单位：华南农业大学林学与风景园林学院
中文关键词：固相微萃取；萃取纤维材料；性信息素；化学物质
英文关键词：solid phase microextraction; extraction of fiber materials; sex pheromones; chemical substances
中文摘要：
摘 要 目前，固相微萃取（Solid phase microextraction，SPME）技术的广泛应用为多个领域发展提供了便利，其优点是方便了现场采样和样品制备，实现了高通量的自动化操作，与气相色谱、质谱等一起联用，便于远程监测。当下依旧需要对该技术的速度、效率和专一性进一步提高。随着SPME技术使用范围的扩大，在昆虫领域方面的研究逐步扩展，从植物源挥发物到昆虫表皮挥发物，再到昆虫性信息素和卵挥发物等方面的应用逐渐增多。本文综述固相微萃取方法及优缺点，以及提取不同昆虫化学物质的最优萃取材料，以期为选择最佳的萃取方法和材料提供参考，从而减少制备样品前处理所需时间，提高样品制备的速度和效率。
英文摘要：
Abstract The increasing application of solid phase microextraction (SPME) technology has improved the development of various fields. The advantages of this technology are that it facilitates on-site sampling and sample preparation, realizes high-throughput automatic operation, and can be combined with gas chromatography and mass spectrometry for remote monitoring. Further improvements to the speed, efficiency and specificity of this technology are, however, still required. SPME has now been applied to plant and insect volatiles, insect sex pheromones and egg volatiles. Here, we summarize the methods, disadvantages and advantages of solid-phase microextraction, and the optimal extraction materials for different insect semio-chemicals. The purpose of this review is to provide a reference for the selection of optimal methods and materials, thereby increasing the speed and efficiency of sample preparation.