褐飞虱和二化螟取食对水稻 植株挥发物的影响
Effects of the volatiles of rice plants fed by Nilaparvata lugens and Chilo suppressalis
刘 旭，吕 静，王满囷
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DOI：10.7679/j.issn.2095-1353.2023.056
作者单位：华中农业大学植物科学技术学院
中文关键词：水稻；褐飞虱；二化螟；诱导挥发物
英文关键词：rice; Nilaparvata lugens; Chilo suppressalis; induced plant volatiles
中文摘要：
摘 要 【目的】 为了探究害虫取食对水稻植物挥发性化合物（Volatile organic compounds, VOCs）的影响。【方法】 利用动态顶空吸附法及GC-MS技术，对褐飞虱Nilaparvata lugens、二化螟Chilo suppressalis 取食水稻植株不同时间的VOCs进行了分离鉴定。【结果】 在本试验条件下，共分离鉴定出水稻VOCs共43种；虫害为害后均未对水稻植株VOCs的种类产生影响，但对水稻植株VOCs的相对含量产生了显著影响，褐飞虱和二化螟取食分别导致3种VOCs（β-水芹烯、癸醛和（E）-5-十四烯）和6种VOCs（2-壬酮、（E）-3-十二烯、十二烷、2,6-二甲基-十一烷、1-癸醇和十八烷）产生显著变化；两种害虫取食的时间对水稻VOCs的相对含量也产生了显著影响。【结论】 害虫取食可对水稻植株VOCs产生显著影响，但VOCs的诱导变化规律与害虫种类及害虫取食时间相关。
英文摘要：
Abstract [Objectives] To investigate the effects of volatiles of rice plants fed by insect pests. [Methods] The volatile compounds induced by the feeding of Nilaparvata lugens and Chilo suppressalis were isolated and identified by dynamic headspace adsorption and Gas Chromatography-Mass Spectrometer. [Results] Under the experimental conditions, a total of 43 kinds of volatiles were isolated and identified from rice plant. After fed by insect pests, the types of volatiles in rice were not different from those in healthy rice, but the relative contents of some volatiles changed significantly. The relative contents of 3 kinds of volatiles were significantly different among rice fed by N. lugens at different time, while the relative contents of 6 kinds of volatiles were significantly different among rice fed by C. suppressalis at different time. The duration of feeding had a significant effect on volatiles in rice plants. [Conclusion] The feeding of insect pests has a significant effect on volatiles in rice plants, and induction and variation of VOCs in rice plants are related to the species of insects and their duration of feeding.