
两种寄主植物对淡足侧沟茧蜂的引诱作用及挥发物成分分析
Attractiveness of two host plants to Microplitis pallidipes and their volatile compounds identification
张彤瑶,蒋杰贤,张 浩,王金彦,尤春梅,陈义娟,季香云
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DOI:10.7679/j.issn.2095-1353.2024.007
作者单位:上海海洋大学水产与生命学院
中文关键词:淡足侧沟茧蜂;玉米;苋菜;甜菜夜蛾;引诱作用;GC-MS
英文关键词:Microplitis pallidipes; maize; amaranth; Spodoptera exigua; attractant effect; GC-MS
中文摘要:
摘 要 【目的】 为了更好的了解寄主植物和甜菜夜蛾Spodoptera exigua幼虫对淡足侧沟茧蜂Microplitis pallidipes趋向行为的影响。【方法】 本研究选用玉米和苋菜两种寄主植物,采用“Y”型嗅觉仪测定了淡足侧沟茧蜂对正常植物和幼虫-植物复合体的行为反应,并对其释放的挥发性物质进行了GC-MS分析与鉴定。【结果】 正常的玉米和苋菜对淡足侧沟茧蜂无明显引诱作用,而幼虫-玉米复合体和幼虫-苋菜复合体均有显著的吸引作用,且幼虫-玉米复合体对该寄生蜂的引诱作用更强。GC-MS检测结果显示在正常玉米中含有22种化合物,主要为烷烃和芳香烃类化合物;幼虫-玉米复合体中有31种化合物,以烷烃和醇类化合物为主;在正常苋菜检测到的16种化合物中,烷烃类化合物含量最高,约占54.75%;而幼虫-苋菜复合体中含有55种化合物,其中以烷烃、芳香烃、烯烃、酸类和醛类物质含量相对较高。另外,与正常植物相比,2-己烯醛、(+)-柠檬烯、α-石竹烯、DMNT和α-雪松烯等化合物仅在幼虫-植物复合体中产生或含量明显升高。【结论】 淡足侧沟茧蜂更倾向于幼虫-玉米复合体,并且玉米和苋菜在遭受甜菜夜蛾取食后,会释放出更多种类的化合物。
英文摘要:
Abstract [Aim] To better understand the effect of host plants and Spodoptera exigua on the tendency behavior of Microplitis pallidipes. [Methods] Maize and amaranth were chosen and the behavioral responses of M. pallidipes to normal plants and larva-plant complex were determined with Y-tube olfactometer, and the volatile compounds were analyzed and identified by GC-MS. [Results] The normal maize and amaranth had no attraction effect on M. pallidipes, but larva-maize complex and larva-amaranth complex exhibited significant attractiveness, and larva-maize complex showed stronger activity than larva-amaranth complex. GC-MS indicated that 22 compounds were detected in normal maize leaves with alkanes and aromatic hydrocarbons as the main constitutes, while 31 compounds were identified in the larva-maize complex and mainly alkanes and alcohols. Similarly, there were 16 compounds in normal amaranth and the content of alkane compounds was the highest with 54.75%. The larva-amaranth complex contained 55 compounds, and alkanes, aromatics, alkenes, acids and aldehydes were relatively high. In addition, some compounds were only produced or increased remarkably in larva-plant complex, such as 2-hexenal, (+) -limonene, α-caryophyllene, DMNT, and α-himachalene. [Conclusion] M. pallidipes were prefer the larva-maize complex, and a wider variety of compounds were released by maize and amaranth after feeding by Spodoptera exigua.