番茄潜叶蛾对四唑虫酰胺的抗性选育及交互抗性研究
Resistance selection of Tuta absoluta (Meyrick) to tetraniliprole and cross-realistic analysis
朱雅婷，张雅蒙，郭晓君，李 娅，张润祥，吴青君，封云涛
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DOI：10.7679/j.issn.2095-1353.2024.020
作者单位：山西农业大学植物保护学院
中文关键词：番茄潜叶蛾；四唑虫酰胺；抗性选育；交互抗性
英文关键词：Tuta absoluta; tetraniliprole; resistance selection; cross-resistance
中文摘要：
摘 要 【目的】 明确番茄潜叶蛾Tuta absoluta（Meyrick）对四唑虫酰胺的抗药性发展趋势及对7种杀虫剂的交互抗性，为四唑虫酰胺的田间应用提供理论依据。【方法】 采用浸叶法，在室内用四唑虫酰胺对番茄潜叶蛾进行抗性选育汰选，评估了抗性发展趋势并研究了交互抗性。【结果】 在室内经过14代11次汰选后，获得了抗性倍数为18.67倍的番茄潜叶蛾四唑虫酰胺抗性品系。番茄潜叶蛾对四唑虫酰胺抗性现实遗传力h2为0.188 5，在致死率为50%-90%选择压力下，预计番茄潜叶蛾对四唑虫酰胺抗性增长10倍需要3.59-8.00代。番茄潜叶蛾抗四唑虫酰胺品系对茚虫威存在明显的交互抗性，抗性倍数为8.89；对甲维盐、苏云金芽孢杆菌（Bt)存在负交互抗性，抗性倍数分别为0.80和0.25；对氯虫苯甲酰胺、多杀菌素、毒死蜱和高效氯氟氰菊酯不存在交互抗性，抗性倍数分别为3.58、2.39、1.80和1.73。【结论】 用四唑虫酰胺防治番茄潜叶蛾存在抗性风险，番茄潜叶蛾四唑虫酰胺抗性品系会与其他药剂产生交互抗性，以上结果可为田间科学使用四唑虫酰胺和合理轮换用药提供理论依据。
英文摘要：
Abstract [Aim] This study aims to investigate the trend in resistance development in Tuta absoluta (Meyrick) to tetraniliprole, and its cross-resistance to seven other insecticides. This research will provide a theoretical basis for the field application of tetraniliprole. [Methods] Resistance of T. absoluta to tetraniliprole was tested in the laboratory using the leaf dipping method. Subsequently, the resistance development trend and degree of cross-resistance were analyzed. [Results] After 11 generations of indoor selection over 14 generations, a strain with a resistance ratio of 18.67 times was obtained. The realized resistance heritability of Tuta absoluta to tetraniliprole was 0.188 5. Tetraniliprole resistance is expected to increase 10-fold in 3.59-8.00 generations under selective pressures of 50%-90%. The resistant strain had obvious cross-resistance to indoxacarb, and the resistance ratio was 8.89. Negative cross-resistance to emamectin benzoate and Bacillus thuringiensis was 0.8 and 0.25-fold, respectively. There was no cross-resistance to chlorantraniliprole, spinosad, chlorpyrifos, or lambda-cyhalothrin, with resistance ratios of 3.58, 2.39, 1.8, and 1.73-fold, respectively. [Conclusion] There is a risk of T. absoluta developing resistance to teraniliprole. Furthermore, the resistant strain of T. absoluta can produce cross-resistance with several other insecticides. These results establish a theoretical basis for the scientific application of teraniliprole in the field and promote the judicious rotation of pesticide use.