基于网络药理学的九香虫水煎液抗肝癌成分及可能机制分析
Network pharmacology analysis of the components and mechanism of Aspongopus chinensis decoction against hepatocellular carcinoma
杨 莎，赵 帅，田 莹，蔡仁莲，陈绪美，郭建军
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DOI：10.7679/j.issn.2095-1353.2024.021
作者单位：贵州大学昆虫研究所
中文关键词：九香虫；水煎液；肝癌；网络药理学；作用机制
英文关键词：Aspongopus chinensis; decoction; hepatocellular carcinoma; network pharmacology; mechanism of action
中文摘要：
摘 要 【目的】 运用网络药理学探究九香虫Aspongopus chinensis水煎液治疗肝癌的成分及可能作用机制。【方法】 在前期测定的九香虫水煎液成分基础上，运用Pubchem、SwissTargetPrediction等数据库收集排名前50的化合物及其可能靶点，GeneCards、Drugbank、DisGeNET等数据库获取肝癌治疗的可能靶点，Cytoscape 3.9.0构建药物-成分-靶点-疾病网络与成分-靶点-通路网络，STRING、cytoHubba等构建PPI网络，Metascap进行KEGG与GO富集分析，得到治疗的关键通路，进而得到关键成分和靶点。【结果】 九香虫水煎液可能通过腺苷等15种成分，作用于EGFR、MAPK1、CCND1等13个靶点，调控20条癌症相关通路发挥其对肝癌的重要治疗作用。【结论】 九香虫水煎液可能通过多成分、多靶点、多通路发挥治疗肝癌的作用。
英文摘要：
Abstract [Aim] To investigate possible components and mechanisms of Aspongopus chinensis decoction against hepatocellular carcinoma using network pharmacology. [Methods] After determining the components of A. chinensis decoction, databases (Pubchem and SwissTargetPrediction) were used to collect the top 50 components and their possible targets. The GeneCards, Drugbank, and DisGeNET databases were used to identify possible targets for hepatocellular carcinoma treatment. Cytoscape 3.9.0 was used to construct a drug-component-target-disease and component-target-pathway network, and the STRING and cytoHubba databases were used to build a PPI network. Finally, the Metascap database was used to conduct KEGG and GO enrichment analyses to obtain the key pathways, components and targets for treatment. [Results] The 15 components (Adenosine, etc.) of A. chinensis decoction may regulate 20 cancer-related pathways through 13 targets (EGFR, MAPK1, CCND1, etc.). This could potentially have a therapeutic effect on hepatocellular carcinoma. [Conclusion] A. chinensis decoction may cure hepatocellular carcinoma through multiple components, targets, and pathways.