熊蜂蜂王肠道代谢物与产卵的相关性研究
The relationship between intestinal metabolites and oviposition in bumblebee queens
刘福罡，郭雨龙，张正义，李继莲
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DOI：10.7679/j.issn.2095-1353.2024.036
作者单位：中国农业科学院蜜蜂研究所
中文关键词：欧洲地熊蜂；兰州熊蜂；产卵；蜂王；代谢组学；差异代谢物
英文关键词：Bombus terrestris; Bombus lantschouensis; oviposition; queen; metabolomics; differential metabolites
中文摘要：
摘 要 【目的】 肠道微生物通过直接或者间接影响宿主的健康，有研究表明肠道微生物能够提高产卵量，但是如何影响产卵具体还不清楚，本研究通过肠道微生物代谢物角度去解释这个问题。【方法】 采用非靶标代谢组学的方法对两种蜂王进行比较，选择欧洲地熊蜂Bombus terrestris产卵蜂王6只、28 d未产卵蜂王6只，兰州熊蜂B. lantschouensis产卵蜂王6只，28 d未产卵蜂王6只，对其肠道内容物进行代谢组学分析。【结果】 产卵欧洲地熊蜂（DQA1）与产卵兰州熊蜂蜂王（LQA1）在脂代谢通路上显著富集；产卵欧洲地熊蜂（DQA1）与28 d未产卵的欧洲地熊蜂（LQW）在半胱氨酸与蛋氨酸代谢通路上显著富集；产卵兰州熊蜂（LQA1）与28 d未产卵的兰州熊蜂蜂王（LQW）在鞘脂代谢通路上显著富集；28 d未产卵的欧洲地熊蜂（DQW）和未产卵的兰州熊蜂蜂王（LQW）没有显著富集的代谢通路。【结论】 在两种产卵蜂王之间，在脂代谢通路上有显著差异。在两种未产卵的蜂王中，欧洲地熊蜂蜂王缺少半胱氨酸与蛋氨酸代谢通路中的半胱氨酸和蛋氨酸两种代谢物质，在未产卵兰州熊蜂蜂王中缺少脂代谢通路中的乙醇胺磷酸酯。
英文摘要：
Abstract [Aim] Gut microbes can directly and indirectly affect host health. Several studies have shown that gut microbes can improve egg production, but the mechanisms involved remains unclear. This study aims to determine how gut microbial metabolites influence oviposition in bumblebees. [Methods] Non-target metabolomics analysis was used to compare the relationship between intestinal metabolites and oviposition in Bombus terrestris and B. lantschouensis queen bees. Six laying queens and six females that were non-ovipositing for 28 d were selected from each species and metabolomics analysis was performed on their intestinal contents. [Results] Enrichment analysis indicated significant enrichment in the lipid metabolism pathways in queens from both the Bombus terrestris (DQA1) and B. lantschouensis (LQA1) ovipositing groups. The cysteine and methionine metabolic pathways were significantly enriched in the Bombus terrestris ovipositing (DQA1) and 28 d non-ovipositing (DQW) groups. Whereas, for B. lantschouensis, the sphingolipid metabolism pathway was significantly enriched in both the LQA1 and 28 d non-ovipositing (LQW) group. There were no significantly enriched metabolic pathways in the 28 d non-ovipositing B terrestris (DQW) or B. lantschouensis (LQW) groups. [Conclusion] There was a significant difference in lipid metabolism pathways between B. terrestris and B. lantschouensis queen bees. Non-ovipositing B. terrestris lacked the L-Cysteine and L-Methionine metabolites in the Cysteine and methionine metabolism pathways. The O-Phosphoethanolamine metabolites in the Cysteine and methionine metabolism pathways were absent in non-ovipositing queen bumblebees.